Weng Lihui, Pan Hui, Chen Weiliam
Department of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, New York 11794-8181, USA.
J Biomed Mater Res A. 2008 May;85(2):352-65. doi: 10.1002/jbm.a.31491.
Self-crosslinkable hydrogels had been formulated from two precursors, partially oxidized hyaluronan (oHA) and gelatin. The physicochemical properties of the resulting hydrogels have been elucidated by instrumental analyses (FTIR, SEM, and rheometry). These hydrogels were highly porous with an average pore size of 60 microm, and evidently, accommodative to cell infiltration. Increasing the oxidation degree of oHA resulted in corresponding increases in hydrogels' storage moduli and decreases in water uptake. Dermal fibroblasts were used to study the cell-hydrogel interactions in vitro. Both the hydrogels and their degradation byproducts are biocompatible as indicated by long-term cell viability assay. In addition, significant amount of cells migrated into the hydrogels and they aligned into highly organized arrays. When cultured with cells, the hydrogels underwent degradation within 4 weeks depending on composition with obvious loss of cohesiveness over time. The good biocompatibility and biodegradability of oHA/gelatin hydrogel were further demonstrated in mice subdermal implantations. Lastly, in vitro and in vivo depositions of extracellular matrix in hydrogels by cells were demonstrated by SEM analyses.
可自交联水凝胶由两种前体物质——部分氧化的透明质酸（oHA）和明胶制成。通过仪器分析（傅里叶变换红外光谱、扫描电子显微镜和流变学）阐明了所得水凝胶的物理化学性质。这些水凝胶具有高度多孔性，平均孔径为60微米，显然有利于细胞浸润。oHA氧化程度的增加导致水凝胶储能模量相应增加，吸水率降低。使用真皮成纤维细胞在体外研究细胞与水凝胶的相互作用。长期细胞活力测定表明，水凝胶及其降解副产物均具有生物相容性。此外，大量细胞迁移到水凝胶中并排列成高度有序的阵列。与细胞一起培养时，水凝胶会在4周内根据组成发生降解，随着时间的推移粘性明显丧失。oHA/明胶水凝胶良好的生物相容性和生物降解性在小鼠皮下植入实验中得到进一步证明。最后，通过扫描电子显微镜分析证明了细胞在水凝胶中的细胞外基质的体外和体内沉积。
