Phillips S C, Turner P C
Department of Biochemistry, Liverpool University.
DNA Seq. 1991;1(6):401-4. doi: 10.3109/10425179109020796.
The U7 snRNP functions in the 3' processing of histone pre-mRNAs of the replication variant class. The U7 snRNA is apparently the only RNA component of this minor snRNP particle. So far, the only U7 snRNA genes that have been isolated are a cluster of 5 from sea urchin. The function of the sea urchin U7 snRNP has been examined by microinjection experiments using Xenopus oocytes and the expression of these sea urchin U7 snRNA genes has been analysed by microinjection into sea urchin eggs. In our studies of U7 snRNA genes in mouse, we have isolated one of only 3 detectable U7 snRNA pseudogenes in the mouse genome and have determined its nucleotide sequence. Sequence comparisons and transcription analysis following microinjection of the gene into Xenopus oocytes supports our assignment of this isolate as a U7 snRNA type II pseudogene.
U7 小核核糖核蛋白（snRNP）在复制变体类组蛋白前体 mRNA 的 3' 加工过程中发挥作用。U7 小核仁 RNA（snRNA）显然是这个较小 snRNP 颗粒的唯一 RNA 组分。到目前为止，唯一已分离出的 U7 snRNA 基因是来自海胆的一组 5 个基因。海胆 U7 snRNP 的功能已通过使用非洲爪蟾卵母细胞的显微注射实验进行了研究，并且这些海胆 U7 snRNA 基因的表达已通过显微注射到海胆卵中进行了分析。在我们对小鼠 U7 snRNA 基因的研究中，我们分离出了小鼠基因组中仅有的 3 个可检测到的 U7 snRNA 假基因之一，并确定了其核苷酸序列。将该基因显微注射到非洲爪蟾卵母细胞后进行的序列比较和转录分析支持我们将这个分离物归为 U7 snRNA II 型假基因。
