Head J F, Chahinian A P, Greengard O
Department of Pediatrics, Mount Sinai School of Medicine, New York, New York 10029.
Cancer Biochem Biophys. 1991 Aug;12(2):127-36.
The enzymic composition of 7 human mesothelioma lines propagated in nude mice was compared with 4 of the original and 15 additional mesotheliomas sampled during the patients' surgery. The xenografts exhibited several-fold higher thymidine kinase (TK), uridine kinase (UK), phosphoserine phosphatase (PSP) and peptidyl proline hydroxylase (PPH) concentrations than the fresh human samples, while their DNA, gamma-glutamyl transpeptidase (GGT) and beta-galactosidase (Bgal) contents remained similar. The volume growth rate of the xenografts (doubling time, DT = 9.23 +/- 1.25 days) was much faster than that of tumors in the human host, and the decline of this rate with increasing nodule size was accompanied by decreases in TK and PSP concentrations. This first quantitative biochemical study of xenografted human neoplasms indicates that 1) pleural mesotheliomas, though preserving their histological characteristics after heterotransplantation, show considerable increases of enzymes in nucleic acid, collagen, and nonessential amino acid synthesis, and that 2) the concentration of TK is a good indicator of the different growth properties of tumors in a mouse rather than in the human host.
将在裸鼠体内传代培养的7株人恶性间皮瘤细胞系的酶组成，与4株原代人恶性间皮瘤以及另外15例患者手术时采集的间皮瘤的酶组成进行了比较。异种移植瘤中胸苷激酶（TK）、尿苷激酶（UK）、磷酸丝氨酸磷酸酶（PSP）和肽基脯氨酸羟化酶（PPH）的浓度比新鲜人标本高几倍，而它们的DNA、γ-谷氨酰转肽酶（GGT）和β-半乳糖苷酶（Bgal）含量则相似。异种移植瘤的体积生长率（倍增时间，DT = 9.23 +/- 1.25天）比人体宿主中的肿瘤快得多，并且随着结节大小增加，该生长率的下降伴随着TK和PSP浓度的降低。这项对异种移植的人类肿瘤的首次定量生化研究表明：1）胸膜间皮瘤尽管在异种移植后保留了其组织学特征，但在核酸、胶原蛋白和非必需氨基酸合成方面的酶有显著增加；2）TK的浓度是小鼠而非人体宿主中肿瘤不同生长特性的良好指标。
