Lindén C J, Johansson L
Dèpartment of Lung Medicine, University Hospital, Lund, Sweden.
Br J Cancer. 1988 Nov;58(5):614-8. doi: 10.1038/bjc.1988.270.
A human malignant pleural mesothelioma was xenografted serially in athymic nude Rowett rats for 27 passages during 33 months. After the two initial passages (P), the take rate during P3-9 was 100% (192/192). The tumour grew progressively during P3-9 in 99% (190/192) and regressed totally in 1% (2/192). The take rate for the tumour xenografted to athymic BALB/c mice was also 100% (17/17) and no regressions were observed. During serial passaging in nude rats, the tumour-volume doubling time (TD) decreased from 6 days in P2 to 3 days in P8-9 (P less than 0.001) and then remained around 3 days during P10-25. A TD of 11 days in P1 (man-mouse) for tumours grown in mice decreased during 10 passages in rats to 4 days (P less than 0.005) when the tumour was transplanted to mice in P11. Light microscopic morphology of the tumour was retained in rats and mice. We believe that our experimental tumour model using the nude rat as a carrier of the xenograft will be useful for studies of human mesothelioma.
将一名人类恶性胸膜间皮瘤在无胸腺裸Rowett大鼠中连续传代移植33个月，共传27代。最初两代传代（P）后，第3 - 9代的接种成功率为100%（192/192）。在第3 - 9代中，99%（190/192）的肿瘤呈进行性生长，1%（2/192）的肿瘤完全消退。移植到无胸腺BALB/c小鼠的肿瘤接种成功率也为100%（17/17），未观察到肿瘤消退。在裸鼠连续传代过程中，肿瘤体积倍增时间（TD）从第2代的6天降至第8 - 9代的3天（P < 0.001），然后在第10 - 25代期间保持在3天左右。在小鼠中生长的肿瘤在第1代（人 - 小鼠）时的TD为11天，在大鼠中传代10次后，当在第11代移植回小鼠时，TD降至4天（P < 0.005）。肿瘤的光镜形态在大鼠和小鼠中得以保留。我们认为，以裸鼠作为异种移植载体的实验性肿瘤模型将有助于人类间皮瘤的研究。
