使用磁共振成像进行细胞追踪。
Cell tracking using magnetic resonance imaging.
作者信息
Hoehn Mathias, Wiedermann Dirk, Justicia Carles, Ramos-Cabrer Pedro, Kruttwig Klaus, Farr Tracy, Himmelreich Uwe
机构信息
In-vivo-NMR Laboratory, Max-Planck-Institute for Neurological Research, Gleueler Strasse 50, D-50931 Köln, Germany.
出版信息
J Physiol. 2007 Oct 1;584(Pt 1):25-30. doi: 10.1113/jphysiol.2007.139451. Epub 2007 Aug 9.
Abstract
Cell tracking by in vivo magnetic resonance imaging (MRI) requires strategies of labelling the cells with MRI contrast agents. The principal routes to achieve efficient cell labelling for neurological applications are discussed with methodological advantages and caveats. Beyond temporo-spatial localization of labelled cells, the investigation of functional cell status is of great interest to allow studies of functional cell dynamics. The two major approaches to reach this goal, use of responsive contrast agents and generation of transgenic cell lines, are discussed.
摘要
通过体内磁共振成像（MRI）进行细胞追踪需要用MRI造影剂标记细胞的策略。本文讨论了在神经学应用中实现高效细胞标记的主要途径及其方法学上的优点和注意事项。除了标记细胞的时空定位外，研究功能性细胞状态对于研究细胞功能动力学也非常重要。本文还讨论了实现这一目标的两种主要方法，即使用响应性造影剂和生成转基因细胞系。
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