对两株类志贺邻单胞菌的O抗原脂多糖进行结构解析，这两株菌与福氏志贺菌6型共享一种型特异性抗原，并与福氏志贺菌属和痢疾志贺菌1型共享共同的1组抗原。
Structural elucidation of the O-antigen lipopolysaccharide from two strains of Plesiomonas shigelloides that share a type-specific antigen with Shigella flexneri 6, and the common group 1 antigen with Shigella flexneri spp and Shigella dysenteriae 1.
作者信息
Linnerborg M, Widmalm G, Weintraub A, Albert M J
机构信息
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.
出版信息
Eur J Biochem. 1995 Aug 1;231(3):839-44.
Sugar and methylation analyses of native polysaccharides together with one-dimensional 1H- and 13C-NMR spectroscopy revealed that the two polysaccharides from strains 22074 and 12254 of Plesiomonas shigelloides are identical. The structure of the polysaccharide from strain 22074 was deduced from a uronic acid degradation and by NMR spectroscopy where heteronuclear multiple bond connectivity and two-dimensional nuclear Overhauser effect spectroscopy experiments established the pentasaccharide repeating unit as-->4)-alpha-D-GalpA-(1-->3)-alpha-D-GlcpNAc-(1-->3)-alpha-L- Rhap-(1-->2)-alpha-L-Rhap-(1-->2)-alpha-L-Rhap-(1-->.
对天然多糖进行的糖和甲基化分析，以及一维¹H-和¹³C-NMR光谱分析表明，弗氏邻单胞菌22074菌株和12254菌株的两种多糖是相同的。通过糖醛酸降解和NMR光谱推断出22074菌株多糖的结构，其中异核多键连接和二维核Overhauser效应光谱实验确定了五糖重复单元为-->4)-α-D-半乳糖醛酸-(1-->3)-α-D-葡萄糖胺-(1-->3)-α-L-鼠李糖-(1-->2)-α-L-鼠李糖-(1-->2)-α-L-鼠李糖-(1-->。
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