Nakagawa Tsuyoshi, Suzuki Takamasa, Murata Satoko, Nakamura Shinya, Hino Takeshi, Maeo Kenichiro, Tabata Ryo, Kawai Tsutae, Tanaka Katsunori, Niwa Yasuo, Watanabe Yuichiro, Nakamura Kenzo, Kimura Tetsuya, Ishiguro Sumie
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, Matsue, Japan.
Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. doi: 10.1271/bbb.70216. Epub 2007 Aug 7.
We made a series of improved Gateway binary vectors (pGWBs) for plant transformation. Fifteen different reporters and tags, sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7, and TAP, were employed. Some vectors carry the 2x35S-Omega promoter for higher-level expression. The kanamycin- and hygromycin-resistant markers are independently available for each of the 43 types of vectors, thus an additional transformation of once-transformed plants can be carried out easily. Their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing. Improved pGWBs should be a powerful tool for transgenic research in plants.
我们构建了一系列用于植物转化的改良型Gateway二元载体（pGWBs）。使用了15种不同的报告基因和标签，即sGFP、GUS、LUC、EYFP、ECFP、G3GFP、mRFP、6xHis、FLAG、3xHA、4xMyc、10xMyc、GST、T7和TAP。一些载体携带2x35S-Omega启动子以实现更高水平的表达。43种载体中的每一种都独立提供了卡那霉素和潮霉素抗性标记，因此可以轻松地对一次转化的植物进行再次转化。它们在大肠杆菌中的小尺寸和高拷贝数使得在质粒制备和测序时更易于操作。改良后的pGWBs应该是植物转基因研究的有力工具。
