Quevedo Marti, Kubalová Ivona, Brun Alexis, Cervela-Cardona Luis, Monte Elena, Strand Åsa
Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, SE-901 87, Sweden.
Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra, Barcelona, 08193, Spain.
Nat Commun. 2025 Jul 15;16(1):6527. doi: 10.1038/s41467-025-61831-w.
Retrograde signalling networks originating in the organelles dictate nuclear gene expression and are essential for control and regulation of cellular energy metabolism. We investigate whether such plastid retrograde signals control nuclear gene expression by altering the chromatin state during the establishment of photosynthetic function in response to light. An Arabidopsis thaliana cell culture provides the required temporal resolution to map four histone modifications during the greening process. We uncover sequential and distinct epigenetic reprogramming events where an epigenetic switch from a histone methylation to an acetylation at photosynthesis-associated loci is dependent on a plastid retrograde signal. The transcription factors VIVIPAROUS1/ABI3-LIKE (VAL1), RELATIVE OF EARLY FLOWERING 6 (REF6) and GOLDEN2-LIKE FACTOR1/2 (GLKs) are linked to the H3K27ac deposition at photosynthesis associated loci that precedes full activation of the photosynthesis genes. Our work demonstrates that retrograde signals play a role in the epigenetic reprogramming essential to the establishment of photosynthesis in plant cells.
源自细胞器的逆行信号网络决定着核基因的表达，对于细胞能量代谢的控制和调节至关重要。我们研究了在响应光照建立光合功能的过程中，此类质体逆行信号是否通过改变染色质状态来控制核基因表达。拟南芥细胞培养物提供了所需的时间分辨率，以绘制绿化过程中四种组蛋白修饰图谱。我们发现了连续且不同的表观遗传重编程事件，其中在光合作用相关位点从组蛋白甲基化到乙酰化的表观遗传转换依赖于质体逆行信号。转录因子VIVIPAROUS1/ABI3-LIKE（VAL1）、EARLY FLOWERING 6的相关蛋白（REF6）和GOLDEN2-LIKE FACTOR1/2（GLKs）与光合作用相关位点上H3K27ac的沉积有关，该沉积先于光合作用基因的完全激活。我们的工作表明，逆行信号在植物细胞光合作用建立所必需的表观遗传重编程中发挥作用。
