Hull J Joe, Kajigaya Ryosuke, Imai Kiyohiro, Matsumoto Shogo
Molecular Entomology Laboratory, RIKEN, Wako, Saitama, Japan.
Biosci Biotechnol Biochem. 2007 Aug;71(8):1993-2001. doi: 10.1271/bbb.70175. Epub 2007 Aug 7.
In most female moths, pheromone biosynthesis activating neuropeptide (PBAN) regulates sex pheromone production by stimulating an influx of extracellular Ca(2+). Little is known about the plasma membrane channel or how the PBAN stimulus is communicated to the channel. Fluorescent Ca(2+) imaging techniques confirmed PBAN-induced Ca(2+) influx in the silkworm, Bombyx mori, and showed that the PBAN response is reduced with repeated stimulation. Compounds known to impact Ca(2+) signaling were examined for their effects on sex pheromone production. These experiments demonstrated that the PBAN signal is likely mediated by a store-operated channel (SOC). SOC blockers, SKF-96365 and 2-aminoethoxydiphenyl borate, abolished sex pheromone production, as did flufenamic acid, a blocker of transient receptor potential (TRP) channels. Thapsigargin mimicked the pheromonotropic effects of PBAN. Similar results were seen when PBAN-induced lipase activity was assayed. Conversely, 1-oleoyl-2-acetyl-sn-glycerol and arachidonic acid, activators of diacylglycerol-dependent Ca(2+) channels, had no effect on bombykol production.
在大多数雌性蛾类中，信息素生物合成激活神经肽（PBAN）通过刺激细胞外Ca(2+)内流来调节性信息素的产生。关于质膜通道或PBAN刺激如何传递到该通道，人们了解甚少。荧光Ca(2+)成像技术证实了PBAN诱导家蚕（Bombyx mori）细胞内Ca(2+)内流，并表明重复刺激会降低PBAN反应。研究了已知影响Ca(2+)信号传导的化合物对性信息素产生的影响。这些实验表明，PBAN信号可能由储存-操作性通道（SOC）介导。SOC阻滞剂SKF-96365和2-氨基乙氧基二苯硼酸以及瞬时受体电位（TRP）通道阻滞剂氟芬那酸均消除了性信息素的产生。毒胡萝卜素模拟了PBAN的促性信息素作用。在检测PBAN诱导的脂肪酶活性时也观察到了类似结果。相反，二酰甘油依赖性Ca(2+)通道的激活剂1-油酰基-2-乙酰基-sn-甘油和花生四烯酸对蚕蛾醇的产生没有影响。
