Májský A
Ustav hematologie a krevní transfúze, Praha.
Cas Lek Cesk. 1991 Oct 18;130(16-17):516-8.
HLA sera were absorbed with erythrocytes with proved HLA antigens and blood cells with HLA-A, B antigens absorbed in vitro from serum. Both types of red cells were 5x to 15x washed in saline and exposed for two hours to the action of 15% chloroquine. The degree of absorption of HLA antibodies and the decline of the score were equal or slightly different after absorption with erythrocytes with own or quinked HLA antigens. Washing in saline or processing of blood cells with chloroquine did not affect the resulting absorption. From the results of the experiment ensues that the link of HLA-A, B antigens is equally firm blood cells isolated from the peripheral blood stream and blood cells where the antigens were linked to them in vitro from serum. This suggests that the view is justified that the majority of HLA-A, B antigens detected in blood cells is secondarily linked from plasma.
HLA血清用具有已证实的HLA抗原的红细胞以及从血清中体外吸收了HLA - A、B抗原的血细胞进行吸收处理。两种类型的红细胞均在盐水中洗涤5至15次，并暴露于15%氯喹作用两小时。用自身或经氯喹处理的具有HLA抗原的红细胞吸收后，HLA抗体的吸收程度和评分下降程度相等或略有不同。在盐水中洗涤或用氯喹处理血细胞不影响最终的吸收效果。从实验结果可以得出，HLA - A、B抗原与从外周血流中分离的血细胞以及在体外从血清中与抗原相连的血细胞的连接同样牢固。这表明认为在血细胞中检测到的大多数HLA - A、B抗原是从血浆中二次连接而来的观点是合理的。
