Korínková P
Vnitr Lek. 1989 Sep;35(9):880-4.
When examining thrombocytic antibodies in serum of patients by the ELISA test concurrently present HLA antibodies were found only in exceptional cases (8), as compared with other tests for the detection of thrombocyte antibodies. One of the possible reasons of this finding could be the mere adsorption of HLA antigens from plasma onto the thrombocyte surface. During the set up of the ELISA test when we work with thrombocytes adhering to the surface of a polystyrene plate and not with a suspension of thrombocytes as in other tests, the spatial arrangement or change of epitopes of HLA antigen could prevent the reaction of HLA antigens with the appropriate antibodies. We tried to test this hypothesis by treating thrombocytes with chloroquine, which is known to remove antigens from the thrombocyte surface, as they are not a firm part of their membrane. Chloroquine actually removed HLA antigens from the thrombocytes as was apparent from: 1. the disappearance of the thrombocyte reactivity with HLA type sera, 2. the inability of thus treated thrombocytes to absorb HLA antibodies from type HLA sera. When resolving the problem of the origin of HLA antigens on thrombocytes we support, based on our results, the view of possible adsorption from plasma, onto the thrombocyte surface. In the discussion the author deals with the possible clinical application of this fact in substitution therapy of refractory patients with thrombocyte concentrates.
通过酶联免疫吸附测定（ELISA）检测患者血清中的血小板抗体时，与其他检测血小板抗体的方法相比，仅在极少数情况下（8例）发现同时存在HLA抗体。这一发现的可能原因之一可能仅仅是血浆中的HLA抗原吸附到了血小板表面。在ELISA检测的设置过程中，我们使用附着在聚苯乙烯板表面的血小板，而不像其他检测那样使用血小板悬液，HLA抗原表位的空间排列或变化可能会阻止HLA抗原与相应抗体发生反应。我们试图通过用氯喹处理血小板来验证这一假设，已知氯喹可以从血小板表面去除抗原，因为它们不是血小板膜的固定组成部分。氯喹实际上从血小板中去除了HLA抗原，这从以下方面明显可见：1. 血小板与HLA分型血清的反应性消失；2. 经如此处理的血小板无法从HLA分型血清中吸收HLA抗体。在解决血小板上HLA抗原的来源问题时，基于我们的结果，我们支持血浆中HLA抗原可能吸附到血小板表面的观点。在讨论中，作者探讨了这一事实在难治性患者血小板浓缩物替代治疗中的可能临床应用。
