Hashimoto K, Hatai M, Yaoi Y
Biology Division, National Cancer Center Research Institute, Tokyo, Japan.
Cell Struct Funct. 1991 Oct;16(5):391-7. doi: 10.1247/csf.16.391.
Human umbilical vein endothelial cells grew well in dishes coated with collagen types I, II, III, or IV. However, the same cells tended to detach themselves from dishes coated with type V collagen, and cell proliferation in these dishes was inhibited. Such anti-adhesive activity was partially retained by heat-denatured type V collagen or by its alpha 1 chain, but not by its alpha 2 chain. Several other cell types did not adhere to the type V collagen substratum even in the presence of 10% serum. The cell types strongly inhibited from adhering by type V collagen included Swiss mouse 3T3 cells and their MSV-transformants, BALB/c 3T3 cells and their methylcholanthrene-transformants, NIH 3T3 cells and their ras-transformants, BHK cells, CHO-9 cells, CHO-K1 cells, and mouse melanoma B16-F10 cells. Using Swiss mouse 3T3, we studied the effects of type V collagen on cell adhesion to fibronectin in serum-free medium. When the culture dishes were coated with a mixture of fibronectin with various concentrations of type V collagen, the adhesion of the cells was inhibited depending on the concentration of type V collagen. The inhibition of cell adhesion by type V collagen was competitively overcome by increased concentrations of fibronectin. The activity that interferes with the effects of fibronectin was retained mainly by the alpha 1 chain of heat-denatured type V collagen.
人脐静脉内皮细胞在包被有I型、II型、III型或IV型胶原蛋白的培养皿中生长良好。然而，同样的细胞往往会从包被有V型胶原蛋白的培养皿上脱离，并且这些培养皿中的细胞增殖受到抑制。热变性的V型胶原蛋白或其α1链部分保留了这种抗黏附活性，但其α2链则没有。即使在含有10%血清的情况下，其他几种细胞类型也不黏附于V型胶原蛋白基质。被V型胶原蛋白强烈抑制黏附的细胞类型包括瑞士小鼠3T3细胞及其MSV转化细胞、BALB/c 3T3细胞及其甲基胆蒽转化细胞、NIH 3T3细胞及其ras转化细胞、BHK细胞、CHO - 9细胞、CHO - K1细胞以及小鼠黑色素瘤B16 - F10细胞。我们使用瑞士小鼠3T3细胞，研究了V型胶原蛋白在无血清培养基中对细胞黏附纤连蛋白的影响。当培养皿包被有不同浓度V型胶原蛋白与纤连蛋白的混合物时，细胞的黏附受到抑制，抑制程度取决于V型胶原蛋白的浓度。增加纤连蛋白的浓度可竞争性地克服V型胶原蛋白对细胞黏附的抑制作用。干扰纤连蛋白作用的活性主要由热变性V型胶原蛋白的α1链保留。
