Fukuda K, Koshihara Y, Oda H, Ohyama M, Ooyama T
Department of Otolaryngology, Kagoshima University, School of Medicine, Japan.
Biochem Biophys Res Commun. 1988 Mar 30;151(3):1060-8. doi: 10.1016/s0006-291x(88)80473-x.
Type V collagen from human placenta remarkably inhibited human umbilical vein endothelial cell (HUVEC) proliferation in a dose-dependent manner when coated on the culture dishes. Other types of collagen (I, III, IV) and fibronectin enhanced HUVEC proliferation under the same conditions. The inhibitory activity of type V collagen was seen not only when it was coated on the dishes, but also when it was directly added into cell culture. The attachment effect of type V collagen did not differ from that of type I collagen. The inhibitory activity is a phenomenon selective for endothelial cells, since type V collagen did not affect the proliferation of human umbilical vein smooth muscle cells, aortic smooth muscle cells, or nasal mucosa fibroblasts.
当包被在培养皿上时，来自人胎盘的V型胶原能以剂量依赖的方式显著抑制人脐静脉内皮细胞（HUVEC）的增殖。在相同条件下，其他类型的胶原（I型、III型、IV型）和纤连蛋白可促进HUVEC的增殖。V型胶原的抑制活性不仅在其包被于培养皿时可见，当直接添加到细胞培养物中时也可见。V型胶原的黏附作用与I型胶原并无差异。这种抑制活性是内皮细胞特有的现象，因为V型胶原并不影响人脐静脉平滑肌细胞、主动脉平滑肌细胞或鼻黏膜成纤维细胞的增殖。
