荧光团标记的肌球蛋白特异性抗体简化了肌纤维表型分析。
Fluorophore-labeled myosin-specific antibodies simplify muscle-fiber phenotyping.
作者信息
Gregorevic Paul, Meznarich Norman A, Blankinship Michael J, Crawford Robert W, Chamberlain Jeffrey S
机构信息
Department of Neurology, Senator Paul D. Wellstone Muscular Dystrophy Cooperative Research Center, University of Washington School of Medicine, 1959 NE Pacific Street, Seattle, Washington 98195-7720, USA.
出版信息
Muscle Nerve. 2008 Jan;37(1):104-6. doi: 10.1002/mus.20877.
Abstract
Skeletal muscles are frequently analyzed for composition of phenotypically distinct myofibers, as a functional determinant. We describe an improved myofiber phenotyping procedure, involving cryosection co-incubation with fluorophore-labeled myosin heavy-chain (MyHC)-isoform-specific antibodies. This technique identifies multiple fiber "types" on a single section, thereby reducing reagents and processing, and offers side-by-side comparison of samples from multiple species including mice. These advances are valuable for studying the physiological attributes of skeletal muscle in health and disease.
摘要
作为一种功能决定因素，骨骼肌常被分析其表型不同的肌纤维组成。我们描述了一种改进的肌纤维表型分析方法，该方法涉及将冷冻切片与荧光团标记的肌球蛋白重链（MyHC）异构体特异性抗体共同孵育。这项技术可在单个切片上识别多种纤维“类型”，从而减少试剂用量和处理步骤，并能对包括小鼠在内的多个物种的样本进行并排比较。这些进展对于研究健康和疾病状态下骨骼肌的生理特性具有重要价值。
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