Yao Bo, Luo Guo-an, Feng Xue, Wang Wei, Chen Ling-xin, Wang Yi-ming
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Lab Chip. 2004 Dec;4(6):603-7. doi: 10.1039/b408422e. Epub 2004 Nov 10.
A novel method based on gravity and electric force driving of cells was developed for flow cytometry and fluorescence activated cell sorting in a microfluidic chip system. In the experiments cells flowed spontaneously under their own gravity in a upright microchip, passed through the detection region and then entered into the sorting electric field one by one at an average velocity of 0.55 mm s(-1) and were fluorescence activated cell sorted (FACS) by a switch-off activation program. In order to study the dynamical and kinematic characteristics of single cells in gravity and electric field of microchannels a physical and numerical module based on Newton's Law of motion was established and optimized. Hydroxylpropylmethyl cellulose (HPMC) was used to minimize cell assembling, sedimentation and adsorption to microchannels. This system was applied to estimate the necrotic and apoptotic effects of ultraviolet (UV) light on HeLa cells by exposing them to UV radiation for 10, 20 or 40 min and the results showed that UV radiation induced membrane damage contributed to the apoptosis and necrosis of HeLa cells.
在微流控芯片系统中，开发了一种基于细胞重力和电力驱动的新方法，用于流式细胞术和荧光激活细胞分选。在实验中，细胞在直立微芯片中在自身重力作用下自发流动，穿过检测区域，然后以平均速度0.55毫米/秒逐一进入分选电场，并通过关闭激活程序进行荧光激活细胞分选（FACS）。为了研究微通道重力和电场中单个细胞的动力学和运动学特性，建立并优化了基于牛顿运动定律的物理和数值模型。使用羟丙基甲基纤维素（HPMC）来减少细胞聚集、沉淀以及对微通道的吸附。通过将HeLa细胞暴露于紫外线（UV）辐射10、20或40分钟，该系统用于评估紫外线对HeLa细胞的坏死和凋亡作用，结果表明紫外线辐射诱导的膜损伤导致了HeLa细胞的凋亡和坏死。
