Sundaresan N R, Saxena V K, Sastry K V H, Nagarajan K, Jain Preeti, Singh Rani, Anish D, Ravindra P V, Saxena M, Ahmed K A
Disease Genetics and Biotechnology Laboratory, Central Avian Research Institute, Izatnagar-243122, Bareilly, India.
Dev Comp Immunol. 2008;32(3):253-64. doi: 10.1016/j.dci.2007.05.011. Epub 2007 Jul 5.
The mechanism of postovulatory follicle (POF) regression in birds is still poorly understood. In the current study, expression of IL-1beta, IL-6, GM-CSF, IFN-gamma, IL-2, IL-4, IL-13, chCXCLi2, chCCLi2, chCCLi4, chCCLi7, IL-10 and TGF-beta2 mRNAs was estimated in regressing POF by semi-quantitative RT-PCR. In addition, the changes in immune cell population, histological and apoptotic changes were also studied in regressing POF. The expression of cytokines (IL-1beta, IL-6, IL-10 and TGF-beta2) and chemokines (chCXCLi2, chCCLi2, chCCLi4 and chCCLi7) was upregulated in POFs, suggesting a role for these molecules in tissue regression. The histological findings suggested a significant infiltration of immune cells, especially heterophils, lymphocytes and macrophages, into the regressing POF. The flow cytometry analysis of lymphocyte subpopulations revealed that CD3(+), CD4(+), CD8(+) and Bu-1(+) lymphocytes were significantly increased during this regression. The significant up-regulation of chemokines might have attracted the immune cells during POF regression. The percentage of apoptotic cells was significantly increased during the regression of POF. The up-regulation of IL-1beta, IL-6, IL-10 and TGF-beta2 and down-regulation of GM-CSF might have induced apoptosis during the POF regression. However, expression of IFN-gamma, IL-2, IL-4 and IL-13 was not significantly altered during POF regression. In conclusion, cytokines appear to play an important role in the regression of POF in chicken. Furthermore, the regression of chicken POF seems to be an inflammatory event similar to luteolysis of the mammalian corpus luteum.
鸟类排卵后卵泡（POF）退化的机制仍未得到充分了解。在本研究中，通过半定量逆转录聚合酶链反应（RT-PCR）评估了退化中的POF中白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、干扰素-γ（IFN-γ）、白细胞介素-2（IL-2）、白细胞介素-4（IL-4）、白细胞介素-13（IL-13）、鸡趋化因子CXCLi2（chCXCLi2）、鸡趋化因子CCLi2（chCCLi2）、鸡趋化因子CCLi4（chCCLi4）、鸡趋化因子CCLi7（chCCLi7）、白细胞介素-10（IL-10）和转化生长因子-β2（TGF-β2）mRNA的表达。此外，还研究了退化中的POF中免疫细胞群体的变化、组织学变化和凋亡变化。细胞因子（IL-1β、IL-6、IL-10和TGF-β2）和趋化因子（chCXCLi2、chCCLi2、chCCLi4和chCCLi7）在POF中的表达上调，表明这些分子在组织退化中起作用。组织学结果表明，免疫细胞，尤其是异嗜性粒细胞、淋巴细胞和巨噬细胞，大量浸润到退化中的POF中。淋巴细胞亚群的流式细胞术分析显示，在这种退化过程中，CD3（+）、CD4（+）、CD8（+）和Bu-1（+）淋巴细胞显著增加。趋化因子的显著上调可能在POF退化过程中吸引了免疫细胞。在POF退化过程中，凋亡细胞的百分比显著增加。IL-1β、IL-6、IL-10和TGF-β2的上调以及GM-CSF的下调可能在POF退化过程中诱导了凋亡。然而，在POF退化过程中，IFN-γ、IL-2、IL-4和IL-13的表达没有显著变化。总之，细胞因子似乎在鸡的POF退化中起重要作用。此外，鸡POF的退化似乎是一个类似于哺乳动物黄体溶解的炎症事件。
