一种用于巨大芽孢杆菌细胞内和细胞外蛋白质生产的蔗糖诱导型启动子系统。
A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium.
作者信息
Biedendieck Rebekka, Gamer Martin, Jaensch Lothar, Meyer Steffen, Rohde Manfred, Deckwer Wolf-Dieter, Jahn Dieter
机构信息
Institute of Microbiology, Technical University Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany.
出版信息
J Biotechnol. 2007 Dec 1;132(4):426-30. doi: 10.1016/j.jbiotec.2007.07.494. Epub 2007 Jul 10.
A sucrose-inducible promoter system (P(sacB)) from Bacillus megaterium was identified using a secretome approach. It was successfully employed for the extracellular production of the homologous levansucrase SacB (4252.4 U l(-1)) and the heterologous green fluorescent protein GFP (7.9 mg g(CDW)(-1)). Mutational analysis of B. megaterium P(sacB) allowed the identification of important promoter elements. The sucrose-inducible promoter provides a useful alternative to the established xylose-inducible promoter system (P(xylA)) for recombinant gene expression in B. megaterium.
采用分泌蛋白质组学方法鉴定了来自巨大芽孢杆菌的蔗糖诱导型启动子系统（P(sacB)）。该系统已成功用于胞外生产同源果聚糖蔗糖酶SacB（4252.4 U l(-1)）和异源绿色荧光蛋白GFP（7.9 mg g(CDW)(-1)）。对巨大芽孢杆菌P(sacB)进行的突变分析有助于确定重要的启动子元件。对于巨大芽孢杆菌中的重组基因表达，蔗糖诱导型启动子为已有的木糖诱导型启动子系统（P(xylA)）提供了一种有用的替代方案。
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