Oguntibeju Oluwafemi O, Meyer Samantha, Aboua Yapo G, Goboza Mediline
Nutrition & Chronic Disease Research Group, Oxidative Stress Research Centre, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, P.O. Box 1906, Bellville 7535, South Africa.
Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, P.O. Box 1906, Bellville 7535, South Africa.
Evid Based Complement Alternat Med. 2016;2016:8934362. doi: 10.1155/2016/8934362. Epub 2016 Jun 15.
Background. Hypoxis hemerocallidea is a native plant that grows in the Southern African regions and is well known for its beneficial medicinal effects in the treatment of diabetes, cancer, and high blood pressure. Aim. This study evaluated the effects of Hypoxis hemerocallidea on oxidative stress biomarkers, hepatic injury, and other selected biomarkers in the liver and kidneys of healthy nondiabetic and streptozotocin- (STZ-) induced diabetic male Wistar rats. Materials and Methods. Rats were injected intraperitoneally with 50 mg/kg of STZ to induce diabetes. The plant extract-Hypoxis hemerocallidea (200 mg/kg or 800 mg/kg) aqueous solution was administered (daily) orally for 6 weeks. Antioxidant activities were analysed using a Multiskan Spectrum plate reader while other serum biomarkers were measured using the RANDOX chemistry analyser. Results. Both dosages (200 mg/kg and 800 mg/kg) of Hypoxis hemerocallidea significantly reduced the blood glucose levels in STZ-induced diabetic groups. Activities of liver enzymes were increased in the diabetic control and in the diabetic group treated with 800 mg/kg, whereas the 200 mg/kg dosage ameliorated hepatic injury. In the hepatic tissue, the oxygen radical absorbance capacity (ORAC), ferric reducing antioxidant power (FRAP), catalase, and total glutathione were reduced in the diabetic control group. However treatment with both doses improved the antioxidant status. The FRAP and the catalase activities in the kidney were elevated in the STZ-induced diabetic group treated with 800 mg/kg of the extract possibly due to compensatory responses. Conclusion. Hypoxis hemerocallidea demonstrated antihyperglycemic and antioxidant effects especially in the liver tissue.
背景。南非星花是一种生长在南部非洲地区的本土植物，因其在治疗糖尿病、癌症和高血压方面的有益药用效果而闻名。目的。本研究评估了南非星花对健康非糖尿病和链脲佐菌素（STZ）诱导的糖尿病雄性Wistar大鼠肝脏和肾脏中的氧化应激生物标志物、肝损伤及其他选定生物标志物的影响。材料与方法。给大鼠腹腔注射50mg/kg的STZ以诱导糖尿病。将植物提取物——南非星花（200mg/kg或800mg/kg）水溶液每日口服给药6周。使用多功能酶标仪分析抗氧化活性，而其他血清生物标志物则使用朗道化学分析仪进行测量。结果。南非星花的两种剂量（200mg/kg和800mg/kg）均显著降低了STZ诱导的糖尿病组的血糖水平。糖尿病对照组和接受800mg/kg治疗的糖尿病组中肝酶活性升高，而200mg/kg剂量改善了肝损伤。在肝组织中，糖尿病对照组的氧自由基吸收能力（ORAC）、铁还原抗氧化能力（FRAP）、过氧化氢酶和总谷胱甘肽降低。然而，两种剂量的治疗均改善了抗氧化状态。接受800mg/kg提取物治疗的STZ诱导糖尿病组中肾脏的FRAP和过氧化氢酶活性升高，可能是由于代偿反应。结论。南非星花表现出降血糖和抗氧化作用，尤其是在肝组织中。
