Kirk Semra J, Ward Theresa H
Immunology Unit, London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
Semin Cell Dev Biol. 2007 Aug;18(4):435-47. doi: 10.1016/j.semcdb.2007.07.007. Epub 2007 Jul 12.
Transport through the secretory pathway begins with COPII regulation of ER export. Driven by the Sar1 GTPase cycle, cytosolic COPII proteins exchange on and off the membrane at specific sites on the ER to regulate cargo exit. Here recent developments in COPII research are discussed, particularly the use of live-cell imaging, which has revealed surprising insights into the coat's role. The seemingly static ER exit sites are in fact highly dynamic, and the ability to visualise trafficking processes in intact living cells has highlighted the adaptable nature of COPII in cargo transport and the emerging roles of auxiliary factors.
通过分泌途径的运输始于COPII对内质网输出的调节。在Sar1 GTPase循环的驱动下，胞质COPII蛋白在内质网特定部位的膜上进行交换，从而调节货物的输出。本文讨论了COPII研究的最新进展，特别是活细胞成像技术的应用，该技术揭示了衣被作用的惊人见解。看似静止的内质网出口位点实际上具有高度动态性，并且在完整活细胞中可视化运输过程的能力突出了COPII在货物运输中的适应性本质以及辅助因子的新作用。
