I型和IV型菌毛生物合成基因的突变会影响桑氏假单胞菌的摆动运动速率。
Mutations in type I and type IV pilus biosynthetic genes affect twitching motility rates in Xylella fastidiosa.
作者信息
De La Fuente Leonardo, Burr Thomas J, Hoch Harvey C
机构信息
Department of Plant Pathology, Cornell University New York State Agricultural Experiment Station, Geneva, NY 14456, USA.
出版信息
J Bacteriol. 2007 Oct;189(20):7507-10. doi: 10.1128/JB.00934-07. Epub 2007 Aug 10.
Abstract
Xylella fastidiosa possesses both type I and type IV pili at the same cell pole. By use of a microfluidic device, the speed of twitching movement by wild-type cells on a glass surface against the flow direction of media was measured as 0.86 (standard error [SE], 0.04) microm min(-1). A type I pilus mutant (fimA) moved six times faster (4.85 [SE, 0.27] microm min(-1)) and a pilY1 mutant moved three times slower (0.28 [SE, 0.03] microm min(-1)) than wild-type cells. Type I pili slow the rate of movement, while the putative type IV pilus protein PilY1 is likely important for attachment to surfaces.
摘要
嗜木质部难养菌在同一细胞极同时拥有I型菌毛和IV型菌毛。通过使用微流控装置，野生型细胞在玻璃表面逆着培养基流动方向进行的颤动运动速度经测量为0.86（标准误差[SE]，0.04）微米/分钟。I型菌毛突变体（fimA）的移动速度比野生型细胞快六倍（4.85[SE，0.27]微米/分钟），而pilY1突变体的移动速度比野生型细胞慢三倍（0.28[SE，0.03]微米/分钟）。I型菌毛会减慢移动速度，而推测的IV型菌毛蛋白PilY1可能对附着于表面很重要。
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