层析相显微镜术。

Tomographic phase microscopy.

作者信息

Choi Wonshik, Fang-Yen Christopher, Badizadegan Kamran, Oh Seungeun, Lue Niyom, Dasari Ramachandra R, Feld Michael S

机构信息

G.R. Harrison Spectroscopy Laboratory, 77 Massachusetts Avenue 6-014, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

出版信息

Nat Methods. 2007 Sep;4(9):717-9. doi: 10.1038/nmeth1078. Epub 2007 Aug 12.

DOI:10.1038/nmeth1078

PMID:17694065

Abstract

We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.

摘要

我们报告了一种使用具有可变照明角度的相移激光干涉显微镜对活细胞和组织中的折射率进行定量三维（3D）映射的技术。我们展示了细胞和多细胞生物体的断层成像以及细胞结构随时间的变化。我们的结果将允许对高分辨率显微镜中样本引起的像差进行定量表征，并在组织光散射方面有多种应用。