Nalayanda Divya D, Puleo Christopher M, Fulton William B, Wang Tza-Huei, Abdullah Fizan
Division of Pediatric Surgery, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA.
Exp Lung Res. 2007 Aug;33(6):321-35. doi: 10.1080/01902140701557754.
In vitro models of the alveolo-pulmonary barrier consist of microvascular endothelial cells and alveolar epithelial cells cultured on opposing sides of synthetic porous membranes. However, these simple models do not reflect the physiological microenvironment of pulmonary cells, wherein cells are exposed to a complex milieu of mechanical and soluble stimuli. In this report, we studied alveolar epithelial (A549) and microvascular endothelial (HMEC-1) cells within varying microfluidic environments as a first step towards building a microfluidic analog of the gas-exchange interface. We fabricated polydimethylsiloxane (PDMS) microdevices for parallel studies of cell growth under multiple flow rates. Cells adhered and proliferated in the microculture chambers for shear stresses up to approximately 2 x 10(-3) dynes/cm(2), corresponding to media turnover rates of approximately 53 seconds. Proliferation of these cells into confluent monolayers and expression of cell-specific markers (SP-A and CD-31) demonstrated successful pulmonary cell culture in microscale devices, a first for alveolar epithelial cells. These results represent the initial steps towards the development of microfluidic analogs of the alveolo-pulmonary barrier and tissue engineering of the lung.
肺泡-肺屏障的体外模型由在合成多孔膜相对两侧培养的微血管内皮细胞和肺泡上皮细胞组成。然而，这些简单模型并未反映肺细胞的生理微环境，在该环境中细胞会暴露于复杂的机械和可溶性刺激物环境中。在本报告中，作为构建气体交换界面微流控模拟物的第一步，我们研究了不同微流控环境中的肺泡上皮（A549）细胞和微血管内皮（HMEC-1）细胞。我们制造了聚二甲基硅氧烷（PDMS）微器件，用于在多种流速下并行研究细胞生长。细胞在微培养室中黏附并增殖，承受的剪切应力高达约2×10⁻³达因/平方厘米，这对应于约53秒的培养基更新率。这些细胞增殖形成汇合单层以及细胞特异性标志物（SP-A和CD-31）的表达，证明了在微尺度器件中成功进行了肺细胞培养，这对肺泡上皮细胞来说尚属首次。这些结果代表了肺泡-肺屏障微流控模拟物开发及肺组织工程的初步步骤。
