Mitchell Martin J, Brescia Aaron I, Smith Stan L, Morgan E David
Department of Biology and Health Services, Edinboro University of Pennsylvania, Edinboro, Pennsylvania, USA.
Arch Insect Biochem Physiol. 2007 Sep;66(1):45-52. doi: 10.1002/arch.20196.
The effects of the natural compound 2-methoxy-1,4-naphthoquinone, isolated from the leaves of Impatiens glandulifera and the synthetic compounds 2-propoxy-1,4-naphthoquinone and 2-isopropoxy-1,4-naphthoquinone on ecdysone 20-monooxygenase (E-20-M) activity were examined in three insect species. Homogenates of wandering stage third instar larvae of Drosophila melanogaster, or abdomens from adult female Aedes aegypti, or fat body or midgut from fifth instar larvae of Manduca sexta were incubated with radiolabelled ecdysone and increasing concentrations (from 1 x 10(-8) to 1 x 10(-3) M) of the three compounds. All three compounds were found to inhibit in a dose-dependent fashion the E-20-M activity in the three insect species. The concentration of these compounds required to elicit a 50% inhibition of this steroid hydroxylase activity in the three insect species examined ranged from approximately 3 x 10(-5) to 7 x 10(-4) M.
研究了从凤仙花叶片中分离得到的天然化合物2-甲氧基-1,4-萘醌以及合成化合物2-丙氧基-1,4-萘醌和2-异丙氧基-1,4-萘醌对三种昆虫蜕皮激素20-单加氧酶（E-20-M）活性的影响。将黑腹果蝇三龄漫游期幼虫的匀浆、埃及伊蚊成年雌蚊的腹部，或烟草天蛾五龄幼虫的脂肪体或中肠与放射性标记的蜕皮激素以及浓度不断增加（从1×10⁻⁸至1×10⁻³ M）的这三种化合物一起孵育。发现所有这三种化合物均以剂量依赖方式抑制这三种昆虫体内的E-20-M活性。在所检测的三种昆虫中，引发这种类固醇羟化酶活性50%抑制所需的这些化合物浓度范围约为3×10⁻⁵至7×10⁻⁴ M。
