Lakhwani Lalit, Tongia Sudheer K, Pal Veerendra S, Agrawal Rajendra P, Nyati Prem, Phadnis Pradeep
Department of Pharmacology, M.G.M Medical College, Indore and MY Group of Hospitals, Indore, India.
Acta Pol Pharm. 2007 May-Jun;64(3):271-6.
Forced swimming test is used to induce a characteristic behavior of immobility in rats, which resembles depression in humans to some extent. We evaluated the effect of omega-3 fatty acids alone as well as compared it with the standard antidepressant therapy with fluoxetine in both acute and chronic studies. In both the studies, rats were divided into 4 groups and subjected to the following drug interventions - Group 1- control: Group 2- fluoxetine in dose of 10 mg/kg subcutaneously 23.5, 5 and 1 h before the test: Group 3- omega-3 fatty acids in dose of 500 mg/kg orally; Group 4- fluoxetine plus omega-3 fatty acids both. In acute study, omega-3 fatty acids were given in single dose 2 h prior to the test while in chronic study omega-3 fatty acids were given daily for a period of 28 days. All animals were subjected to a 15-min pretest followed 24 h later by a 5-min test. A time sampling method was used to score the behavioral activity in each group. The results revealed that in acute study, omega-3 fatty acids do not have any significant effect in forced swimming test. However, in chronic study, omega-3 fatty acids affect the immobility and swimming behavior significantly when compared with control (p < 0.01) without any significant effect on climbing behavior and the efficacy of combination of omega-3 fatty acids and fluoxetine is significantly more than that of fluoxetine alone in changing the behavioral activity of rats in forced swimming test. It leads to the conclusion that omega-3 fatty acids have antidepressant activity per se, and the combination of fluoxetine and omega-3 fatty acids has more antidepressant efficacy than fluoxetine alone in forced swimming test in Wistar rats.
强迫游泳试验用于诱导大鼠出现一种静止不动的特征性行为，这种行为在一定程度上类似于人类的抑郁状态。在急性和慢性研究中，我们评估了单独使用ω-3脂肪酸的效果，并将其与使用氟西汀的标准抗抑郁疗法进行了比较。在这两项研究中，大鼠被分为4组，并接受以下药物干预——第1组为对照组；第2组在试验前23.5、5和1小时皮下注射剂量为10 mg/kg的氟西汀；第3组口服剂量为500 mg/kg的ω-3脂肪酸；第4组同时使用氟西汀和ω-3脂肪酸。在急性研究中，ω-3脂肪酸在试验前2小时单次给药，而在慢性研究中，ω-3脂肪酸连续28天每日给药。所有动物均先进行15分钟的预试验，24小时后进行5分钟的试验。采用时间抽样方法对每组的行为活动进行评分。结果显示，在急性研究中，ω-3脂肪酸在强迫游泳试验中没有任何显著效果。然而，在慢性研究中，与对照组相比，ω-3脂肪酸对静止不动和游泳行为有显著影响（p<0.01），对攀爬行为没有显著影响，并且在强迫游泳试验中，ω-3脂肪酸与氟西汀联合使用改变大鼠行为活动的效果明显优于单独使用氟西汀。由此得出结论，ω-3脂肪酸本身具有抗抑郁活性，在Wistar大鼠的强迫游泳试验中，氟西汀与ω-3脂肪酸联合使用比单独使用氟西汀具有更强的抗抑郁效果。
