Nagahama M, Kobayashi K, Ochi S, Sakurai J
Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan.
FEMS Microbiol Lett. 1991 Nov 1;68(1):41-4. doi: 10.1016/0378-1097(91)90392-n.
An enzyme-linked immunosorbent assay (ELISA) with antibodies specific to beta, epsilon and iota ib toxins of Clostridium perfringens was developed to detect beta, epsilon and iota ib toxins, respectively. The ELISA was sensitive enough to detect as little as 1.0 ng/ml of purified beta and iota ib toxins and 0.1 ng/ml of purified epsilon toxin. By means of the ELISA method, 192 isolates of C. perfringens from food samples in Japan and Thailand, and 58 isolates from patients suffering from gas gangrene or gastroenteritis were examined. One isolate from food samples in Japan, three from food samples in Thailand and five from stools of patients with gastroenteritis were C. perfringens type D. One type B and one type C were detected from the stools of patients with gastroenteritis.
开发了一种酶联免疫吸附测定（ELISA），使用针对产气荚膜梭菌β、ε和iota ib毒素的特异性抗体，分别检测β、ε和iota ib毒素。该ELISA灵敏度足以检测低至1.0 ng/ml的纯化β和iota ib毒素以及0.1 ng/ml的纯化ε毒素。通过ELISA方法，检测了来自日本和泰国食品样本的192株产气荚膜梭菌分离株，以及58株来自气性坏疽或肠胃炎患者的分离株。来自日本食品样本的1株分离株、来自泰国食品样本的3株分离株和来自肠胃炎患者粪便的5株分离株为D型产气荚膜梭菌。从肠胃炎患者的粪便中检测到1株B型和1株C型。
