Hurkadli K S, Joseph R, Garde S V, Sheth A R
Institute for Research in Reproduction (ICMR), Bombay, India.
Indian J Exp Biol. 1991 Aug;29(8):715-20.
Using specific polyclonal antibodies generated against a 13 Kd human testicular inhibin, immunocytochemical localization was carried out in epididymis of intact and castrated marmoset monkey and rat epididymis. Inhibin was found to be present in the cytoplasm of epithelial cells of caput, corpus and cauda epididymis. The intensity of staining and pattern of distribution did not change following castration. Further, the in vitro biosynthesis of inhibin studied by incorporating 3H-leucine and precipitating it with specific antibody indicated maximum biosynthesis in the corpus epididymis in case of marmosets and cauda in case of rats. Following castration in rats, the epididymal tissue still retained the capacity to biosynthesize inhibin. These studies indicate that marmoset and rat epididymis are capable of biosynthesizing/absorbing inhibin and whose synthesis does not depend on androgens.
利用针对一种13 Kd人睾丸抑制素产生的特异性多克隆抗体，在完整和去势的狨猴附睾以及大鼠附睾中进行了免疫细胞化学定位。发现抑制素存在于附睾头、体和尾的上皮细胞胞质中。去势后染色强度和分布模式没有改变。此外，通过掺入3H-亮氨酸并用特异性抗体沉淀来研究抑制素的体外生物合成，结果表明，狨猴附睾体和大鼠附睾尾的生物合成量最大。大鼠去势后，附睾组织仍保留生物合成抑制素的能力。这些研究表明，狨猴和大鼠附睾能够生物合成/吸收抑制素，且其合成不依赖雄激素。
