Garde S V, Sheth A R, Zaveri B J, Shah J, Hinduja I
Institute for Research in Reproduction, Bombay, India.
Indian J Exp Biol. 1991 Oct;29(10):889-96.
Using polyclonal antibodies generated against human seminal plasma inhibin (10.5 KDa), immunocytochemical localization was carried out in paraffin embedded tissue sections of human endometrial biopsies obtained at various phases of the menstrual cycle. A positive reaction which indicated the presence of inhibin was characterized by the presence of golden yellow or brown colour in the cytoplasm of epithelial cells that formed the glands as well as the luminal lining. The stromal cells however, showed negative staining. In early proliferative phase, the endometrial glands exhibited weak positive staining for inhibin which gradually increased and was intense in late follicular and early secretory phases. The intensity of the staining although was not diminished in the glandular epithelium in the mid as well as late secretory phases, the number of cells showing positive staining appeared to be reduced. Incubation of endometrial biopsies in vitro with labelled amino acid and immunoprecipitation of newly synthesized protein with specific antibodies to inhibin indicated that endometrium is capable of de novo synthesizing inhibin. The above results suggest that endometrium is an extra ovarian source of inhibin and the possible role of endometrial peptide in sperm fertilizing capabilities as well as in pre and post implantation events is suggested.
利用针对人精浆抑制素（10.5千道尔顿）产生的多克隆抗体，对在月经周期不同阶段获取的人子宫内膜活检石蜡包埋组织切片进行免疫细胞化学定位。表明存在抑制素的阳性反应的特征是，形成腺体以及腔内膜的上皮细胞胞质中出现金黄色或棕色。然而，基质细胞呈阴性染色。在增殖早期，子宫内膜腺体对抑制素呈弱阳性染色，其染色逐渐增强，在卵泡晚期和分泌早期染色强烈。尽管在分泌中期和晚期腺上皮中的染色强度未减弱，但显示阳性染色的细胞数量似乎减少。用标记氨基酸对子宫内膜活检进行体外孵育，并用针对抑制素的特异性抗体对新合成的蛋白质进行免疫沉淀，结果表明子宫内膜能够从头合成抑制素。上述结果表明，子宫内膜是抑制素的卵巢外来源，并提示子宫内膜肽在精子受精能力以及植入前和植入后事件中可能发挥的作用。
