Simark-Mattsson Charlotte, Emilson Claes-Göran, Håkansson Eva Grahn, Jacobsson Catharina, Roos Kristian, Holm Stig
Department of Cariology, Institute of Odontology, The Sahlgrenska Academy at Göteborg University, SE-405 30 Göteborg, Sweden.
Eur J Oral Sci. 2007 Aug;115(4):308-14. doi: 10.1111/j.1600-0722.2007.00458.x.
In order to assess whether naturally occurring oral lactobacilli have probiotic properties, lactobacilli were isolated from saliva and plaque from children and adolescents, with or without caries lesions. The interference capacities of these lactobacilli were investigated against a panel of 13 clinical isolates and reference strains of Streptococcus mutans and Streptococcus sobrinus, as well as against the subject's autologous mutans streptococci, using the agar-overlay technique. Lactobacillus-mediated inhibition differed significantly between the three subject groups (no caries, arrested caries, or active caries), demonstrating increased inhibition in subjects without present or previous caries experience compared to subjects with arrested caries or subjects presenting with frank lesions. Lactobacilli from subjects lacking S. mutans inhibited the growth of the test panel of mutans streptococci significantly better than lactobacilli from subjects who were colonized. Furthermore, subjects without caries experience harbored lactobacilli that more effectively repressed the growth of their autologous mutans streptococci. Twenty-three Lactobacillus spp. completely inhibited the growth of all mutans streptococci tested. Species with maximum interference capacity against mutans streptococci included Lactobacillus paracasei, Lactobacillus plantarum, and Lactobacillus rhamnosus. Naturally occurring oral lactobacilli significantly inhibited the growth of both test strains of mutans streptococci and the subject's autologous mutans streptococci in vitro, and this effect was more pronounced in caries-free subjects.
为了评估天然存在的口腔乳酸杆菌是否具有益生菌特性，从有或无龋损的儿童和青少年的唾液及牙菌斑中分离出乳酸杆菌。使用琼脂覆盖技术，研究了这些乳酸杆菌对一组13种变形链球菌和远缘链球菌的临床分离株及参考菌株的干扰能力，以及对受试者自身变形链球菌的干扰能力。乳酸杆菌介导的抑制作用在三个受试者组（无龋、静止龋或活动龋）之间存在显著差异，表明与有静止龋的受试者或有明显龋损的受试者相比，无当前或既往龋经验的受试者的抑制作用增强。来自缺乏变形链球菌的受试者的乳酸杆菌比来自已定植受试者的乳酸杆菌能更显著地抑制变形链球菌测试组的生长。此外，无龋经验的受试者体内的乳酸杆菌能更有效地抑制其自身变形链球菌的生长。23种乳酸杆菌完全抑制了所有测试的变形链球菌的生长。对变形链球菌具有最大干扰能力的菌种包括副干酪乳杆菌、植物乳杆菌和鼠李糖乳杆菌。天然存在的口腔乳酸杆菌在体外能显著抑制变形链球菌测试菌株和受试者自身变形链球菌的生长，且这种作用在无龋受试者中更为明显。
