Schulmerich Matthew V, Dooley Kathryn A, Vanasse Thomas M, Goldstein Steven A, Morris Michael D
University of Michigan, Department of Chemistry, Ann Arbor, Michigan 48109-1055, USA.
Appl Spectrosc. 2007 Jul;61(7):671-8. doi: 10.1366/000370207781393307.
Different spatial separations between an illumination ring and a bundle of 50 collection fibers focused to collect light in the center of the ring were used to investigate the recovery of subsurface Raman spectra. The depth of Raman signal recovery and the preservation of spatial information in the recovered signal were investigated using polymer blocks stacked in different geometries. The illumination rings were then combined into a single data set to increase variation in the signal. Multivariate data analysis was used to recover the Raman spectra of the subsurface component. The Raman spectrum of a Delrin target was recoverable at depths up to 22.6 mm of overlying Teflon. Spatial information was lost at approximately 6.5 mm below the Teflon surface. The same protocols were used to recover canine bone spectra transcutaneously at depths up to 5 mm below the skin's surface. The recovered bone spectra were validated by exposed bone measurements.
利用照明环与一束聚焦于环中心以收集光的50根收集光纤之间的不同空间间隔，研究了地下拉曼光谱的恢复情况。使用堆叠成不同几何形状的聚合物块，研究了拉曼信号恢复的深度以及恢复信号中空间信息的保留情况。然后将照明环组合成一个单一数据集，以增加信号的变化。采用多元数据分析来恢复地下成分的拉曼光谱。在覆盖的聚四氟乙烯厚度达22.6毫米的深度处，可恢复Delrin靶标的拉曼光谱。在聚四氟乙烯表面以下约6.5毫米处，空间信息丢失。采用相同的方案经皮恢复犬骨光谱，深度可达皮肤表面以下5毫米。通过暴露的骨骼测量对恢复的骨光谱进行了验证。
