Tseng Li-Ho, Li Mei-Hui, Tsai Shinn-Shyong, Lee Chia-Wei, Pan Min-Hsiung, Yao Wei-Jen, Hsu Ping-Chi
Department of Safety, Health and Environmental Engineering, National Kaohsiung First University of Science and Technology, No. 2, Jhuoyue Road, Nanzih District, Kaohsiung City 811, Taiwan.
Chemosphere. 2008 Jan;70(4):640-7. doi: 10.1016/j.chemosphere.2007.06.078. Epub 2007 Aug 14.
Decabrominated diphenyl ether (PBDE 209) is the second most used brominated flame retardant (BFRs). Many studies have shown that some of the BFRs act as endocrine disruptors via alterations in thyroid hormone homeostasis and affect development. Little is known about the effect of prenatal exposure to PBDE 209 on the development in male offspring. Using a CD-1 mouse model, we attempt to estimate the possible effect of in utero exposure to PBDE 209 on thyroid hormone and hepatic enzymes activities in male offspring. Pregnant mice were administered different doses of PBDE 209 (10, 500, and 1500 mg/kg/day) or corn oil for controls per gavage from gestational days 0-17. In adult male offspring whose mothers had been treated with 1500 mg/kg of PBD 209, hepatic enzyme activity of S9 7-ethoxyresorufin O-deethylase (EROD) was weak but significantly increased (54%). However, no significant changes were observed in S9 4-nitrophenol uridinediphosphate-glucuronosyltransferase (UDPGT) in any of the treatment groups. Serum triiodothyronine (T3) was found to have decreased significantly (ca. 21% both 10 mg/kg and 1500 mg/kg) in offspring, but not thyroxine (T4). Histopathological examination revealed that prenatal exposure of PBDE 209 might be related with cell swelling of hepatocytes in male offspring and there were mild changes in the thyroid glands in 1500 mg/kg group. These data demonstrate that PBDE 209 is likely an endocrine disrupter in male mice following exposure during development. Further studies using environmentally relevant doses are needed for hazard identification.
十溴二苯醚（PBDE 209）是第二大常用的溴化阻燃剂（BFRs）。许多研究表明，一些溴化阻燃剂通过改变甲状腺激素稳态而充当内分泌干扰物，并影响发育。关于产前暴露于PBDE 209对雄性后代发育的影响知之甚少。使用CD-1小鼠模型，我们试图评估子宫内暴露于PBDE 209对雄性后代甲状腺激素和肝酶活性的可能影响。从妊娠第0天至17天，通过灌胃给怀孕小鼠施用不同剂量的PBDE 209（10、500和1500毫克/千克/天）或玉米油作为对照。在其母亲接受1500毫克/千克PBDE 209处理的成年雄性后代中，S9 7-乙氧基异吩唑酮O-脱乙基酶（EROD）的肝酶活性较弱，但显著增加（54%）。然而，在任何处理组中，S9 4-硝基苯酚尿苷二磷酸葡萄糖醛酸转移酶（UDPGT）均未观察到显著变化。发现后代血清三碘甲状腺原氨酸（T3）显著降低（10毫克/千克和1500毫克/千克组均约降低21%），但甲状腺素（T4）未降低。组织病理学检查显示，产前暴露于PBDE 209可能与雄性后代肝细胞肿胀有关，并且在1500毫克/千克组中甲状腺有轻微变化。这些数据表明，PBDE 209在发育过程中暴露后可能是雄性小鼠的内分泌干扰物。需要使用与环境相关的剂量进行进一步研究以确定危害。
