从人类粪便样本中增强隐孢子虫囊合子的浓缩和分离。
Enhanced concentration and isolation of Cyclospora cayetanensis oocysts from human fecal samples.
作者信息
Riner Diana K, Mullin Andrew S, Lucas Sasha Y, Cross John H, Lindquist H D Alan
机构信息
Pegasus Technical Services Inc., 46 E. Hollister St. Cincinnati, OH 45219, United States.
出版信息
J Microbiol Methods. 2007 Oct;71(1):75-7. doi: 10.1016/j.mimet.2007.06.021. Epub 2007 Jul 18.
PMID:17698229
Abstract
Cyclospora cayetanensis is the causative agent of cyclosporiasis, an emerging infectious disease. We present a new method for the purification of C. cayetanensis oocysts from feces using a modified detachment solution and Renocal-sucrose gradient sedimentation. This method yields oocysts free from adherent fecal debris and amenable to processing using flow cytometry.
摘要
卡耶塔环孢子球虫是环孢子虫病的病原体，这是一种新出现的传染病。我们提出了一种新方法，使用改良的分离溶液和雷诺钙-蔗糖梯度沉降法从粪便中纯化卡耶塔环孢子球虫卵囊。该方法可产生不含附着粪便残渣的卵囊，并且适合使用流式细胞术进行处理。
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