Rico Eduardo Pacheco, Rosemberg Denis Broock, Senger Mario Roberto, de Bem Arizi Marcelo, Dias Renato Dutra, Souto André Arigony, Bogo Maurício Reis, Bonan Carla Denise
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul. Rua Ramiro Barcelos 2600-Anexo, 90035-003 Porto Alegre, RS, Brazil.
Neurochem Int. 2008 Jan;52(1-2):290-6. doi: 10.1016/j.neuint.2007.06.034. Epub 2007 Jul 4.
Alcohol abuse is an acute health problem throughout the world and alcohol consumption is linked to the occurrence of several pathological conditions. Here we tested the acute effects of ethanol on NTPDases (nucleoside triphosphate diphosphohydrolases) and 5'-nucleotidase in zebrafish (Danio rerio) brain membranes. The results have shown a decrease on ATP (36.3 and 18.4%) and ADP (30 and 20%) hydrolysis after 0.5 and 1% (v/v) ethanol exposure during 60 min, respectively. In contrast, no changes on 5'-nucleotidase activity were observed in zebrafish brain membranes. Ethanol in vitro did not alter ATP and ADP hydrolysis, but AMP hydrolysis was inhibited at 0.5, and 1% (23 and 28%, respectively). Acetaldehyde in vitro, in the range 0.5-1%, inhibited ATP (40-85%) and ADP (28-65%) hydrolysis, whereas AMP hydrolysis was reduced (52, 58 and 64%) at 0.25, 0.5 and 1%, respectively. Acetate in vitro did not alter these enzyme activities. Semi-quantitative expression analysis of NTPDase and 5'-nucleotidase were performed. Ethanol treatment reduced NTPDase1 and three isoforms of NTPDase2 mRNA levels. These findings demonstrate that acute ethanol intoxication may influence the enzyme pathway involved in the degradation of ATP to adenosine, which could affect the responses mediated by adenine nucleotides and nucleosides in zebrafish central nervous system.
酒精滥用是一个全球性的严重健康问题，饮酒与多种病理状况的发生有关。在此，我们测试了乙醇对斑马鱼（Danio rerio）脑膜中NTPDases（核苷三磷酸二磷酸水解酶）和5'-核苷酸酶的急性影响。结果显示，在60分钟内分别暴露于0.5%和1%（v/v）乙醇后，ATP（分别降低36.3%和18.4%）和ADP（分别降低30%和20%）的水解减少。相比之下，斑马鱼脑膜中的5'-核苷酸酶活性未观察到变化。体外乙醇并未改变ATP和ADP的水解，但在0.5%和1%时AMP水解受到抑制（分别为23%和28%）。体外0.5%-1%范围内的乙醛抑制ATP（40%-85%）和ADP（28%-65%）的水解，而在0.25%、0.5%和1%时AMP水解分别减少（52%、58%和64%）。体外醋酸盐未改变这些酶的活性。对NTPDase和5'-核苷酸酶进行了半定量表达分析。乙醇处理降低了NTPDase1和NTPDase2三种同工型的mRNA水平。这些发现表明，急性乙醇中毒可能影响参与ATP降解为腺苷的酶途径，这可能会影响斑马鱼中枢神经系统中腺嘌呤核苷酸和核苷介导的反应。
