Schneider Ana Claudia Reis, Rico Eduardo Pacheco, de Oliveira Diogo Losch, Rosemberg Denis Broock, Guizzo Ranieli, Meurer Fábio, da Silveira Themis Reverbel
Programa de Pós-Graduação: Ciências em Gastroenterologia e Hepatologia, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .
Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense-UNESC , Criciúma, Brazil .
Biores Open Access. 2016 Jan 1;5(1):1-5. doi: 10.1089/biores.2015.0026. eCollection 2016.
Ethanol is a widely consumed drug, which acts on the central nervous system to induce behavioral alterations ranging from disinhibition to sedation. Recent studies have produced accumulating evidence for the therapeutic role of probiotic bacteria in behavior. We aimed to investigate the effect of Lactobacillus rhamnosus GG (LGG) on the behavior of adult zebrafish chronically exposed to ethanol. Adult wild-type zebrafish were randomly divided into four groups, each containing 15 fish. The following groups were formed: Control (C), received unsupplemented feed during the trial period; Probiotic (P), fed with feed supplemented with LGG; Ethanol (E), received unsupplemented feed and 0.5% of ethanol directly added to the tank water; and Probiotic+Ethanol (P+E), group under ethanol exposure (0.5%) and fed with LGG supplemented feed. After 2 weeks of exposure, the novel tank test was used to evaluate fish behavior, which was analyzed using computer-aided video tracking. LGG alone did not alter swimming behavior of the fish. Ethanol exposure led to robust behavioral effects in the form of reduced anxiety levels, as indicated by increased vertical exploration and more time spent in the upper region of the novel tank. The group exposed to ethanol and treated with LGG behaved similarly to animals exposed to ethanol alone. Taken together, these results show that zebrafish behavior was not altered by LGG per se, as seen in murine models. This was the first study to investigate the effects of a probiotic diet on behavior after a chronic ethanol exposure.
乙醇是一种广泛使用的药物，作用于中枢神经系统，可引起从行为抑制解除到镇静等一系列行为改变。最近的研究不断积累证据，表明益生菌在行为方面具有治疗作用。我们旨在研究鼠李糖乳杆菌GG（LGG）对长期暴露于乙醇的成年斑马鱼行为的影响。成年野生型斑马鱼被随机分为四组，每组15条鱼。分组如下：对照组（C），在试验期间给予未添加任何物质的饲料；益生菌组（P），投喂添加了LGG的饲料；乙醇组（E），给予未添加任何物质的饲料，并在鱼缸水中直接添加0.5%的乙醇；益生菌+乙醇组（P+E），暴露于乙醇（0.5%）环境中并投喂添加LGG的饲料。暴露2周后，采用新鱼缸试验评估鱼类行为，并用计算机辅助视频跟踪进行分析。单独使用LGG不会改变鱼的游泳行为。乙醇暴露导致了明显的行为效应，表现为焦虑水平降低，这通过垂直探索增加以及在新鱼缸上部区域停留时间延长得以体现。暴露于乙醇并接受LGG处理的组与单独暴露于乙醇的动物表现相似。综上所述，这些结果表明，与在小鼠模型中观察到的情况一样，LGG本身不会改变斑马鱼的行为。这是第一项研究慢性乙醇暴露后益生菌饮食对行为影响的研究。
