Näher H, Schüle T, Hartmann M, Petzoldt D
Hautklinik, Ruprecht-Karls-Universität Heidelberg.
Hautarzt. 1991 Nov;42(11):704-6.
The infection of cells of the epidermis with HIV can still not be taken as proved, since the demonstration of HIV in Langerhans cells has not been universally accepted. In our study, the polymerase chain reaction was used to look for proviral HIV-DNA in epidermal cells collected by the suction blister technique. The primers flanked sequences of the gp41-encoding region of the env gene and hybridized with highly conserved HIV-DNA sequences. Amplified sequences were detected by the dot-blot technique. The PCR revealed HIV-DNA in epidermals cells of four HIV patients. In addition, HIV-DNA was detected in the mononuclear cells of the blood in the same patients by the PCR. These results strongly support the notion that in addition to blood cells, epidermal cells of HIV patients are also infected with HIV.
艾滋病毒对表皮细胞的感染仍不能被视为已得到证实，因为在朗格汉斯细胞中发现艾滋病毒尚未得到普遍认可。在我们的研究中，采用聚合酶链反应在通过吸疱技术收集的表皮细胞中寻找前病毒型艾滋病毒DNA。引物位于env基因的gp41编码区序列两侧，并与高度保守的艾滋病毒DNA序列杂交。通过斑点印迹技术检测扩增序列。聚合酶链反应在4例艾滋病毒患者的表皮细胞中检测到了艾滋病毒DNA。此外，通过聚合酶链反应在同一患者的血液单核细胞中也检测到了艾滋病毒DNA。这些结果有力地支持了这一观点，即除血细胞外，艾滋病毒患者的表皮细胞也感染了艾滋病毒。
