Gokcimen A, Cim A, Tola H T, Bayram D, Kocak A, Ozgüner F, Ayata A
Department of Histology and Embryology, Suleyman Demirel University, Isparta, Turkey.
Hum Exp Toxicol. 2007 Jun;26(6):519-25. doi: 10.1177/0960327107076885.
The aim of this study was to compare the possible protective effects of N-acetylcysteine (NAC), caffeic acid (CAPE) and vitamin E (Vit-E) on doxorubicin-induced hepatotoxicity. Thirty-two male Wistar albino rats, weighing between 250 and 350 g were supplied and randomly divided into five groups. Animals in study groups were pretreated with a single dose of doxorubicin (Dox), which was administered intraperitoneally (i.p.). Control group (Group I) was treated with intraperitoneal saline injection. Group II did not received any antioxidant agent after the injection. Group III and Group IV were given CAPE and intraperitoneal vitamin E injection for eight days, respectively. Group V received NAC for eight days. The study was finished after 10 days. Tissue samples were collected from all animals and histopathological examination was performed. There was statistically significant difference between the experiment groups and controls by means of mononuclear cell infiltration and diameters of hepatic sinusoid, terminal hepatic venule (central vein) and portal area (portal canal). Changes related with hepatocellular damage were more prominent, whereas there was no significant difference between Dox and NAC given groups histopathologically. It was observed that structural changes were regressed after CAPE administration. However, this recovery was more prominent in vitamin E given group. These findings suggest that Dox induced liver damage could be efficiently reversed by vitamin E administration. It has been found that CAPE, but not NAC has protective effects on Dox-induced hepatocellular damage.
本研究的目的是比较N-乙酰半胱氨酸（NAC）、咖啡酸（CAPE）和维生素E（Vit-E）对阿霉素诱导的肝毒性可能的保护作用。提供了32只体重在250至350克之间的雄性Wistar白化大鼠，并将其随机分为五组。研究组的动物预先腹腔注射（i.p.）单剂量的阿霉素（Dox）。对照组（I组）接受腹腔注射生理盐水。II组在注射后未接受任何抗氧化剂。III组和IV组分别连续八天给予CAPE和腹腔注射维生素E。V组连续八天接受NAC。10天后研究结束。从所有动物收集组织样本并进行组织病理学检查。通过单核细胞浸润以及肝血窦、终末肝小静脉（中央静脉）和门管区（门静脉管）的直径，实验组与对照组之间存在统计学上的显著差异。与肝细胞损伤相关的变化更为突出，而给予Dox和NAC的组在组织病理学上没有显著差异。观察到给予CAPE后结构变化有所消退。然而，这种恢复在给予维生素E的组中更为明显。这些发现表明，给予维生素E可有效逆转Dox诱导的肝损伤。已发现CAPE对Dox诱导的肝细胞损伤有保护作用，而NAC则没有。
