Hayashi Y, Makino H, Shikata K, Haramoto T, Yamasaki Y, Kumagai I, Ota Z
Third Department of Internal Medicine, Okayama University Medical School, Japan.
J Diabet Complications. 1991 Apr-Sep;5(2-3):195-6. doi: 10.1016/0891-6632(91)90072-w.
We measured serum and urinary concentrations of type IV collagen by radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) in diabetics. Serum and urinary concentrations of type IV collagen measured by RIA and ELISA were increased compared to those of control subjects. In diabetics with macroproteinuria or with renal insufficiency, serum and urinary concentrations of type IV collagen were higher than in diabetics without nephropathy or with early renal damage as determined by the presence of microproteinuria. These results suggest that serum and urinary concentrations of type IV collagen are increased in patients with advanced diabetic nephropathy. These increases may indicate that alteration of basement membrane metabolism has occurred in diabetics.
我们通过放射免疫分析法（RIA）和酶联免疫吸附测定法（ELISA）测定了糖尿病患者血清和尿液中IV型胶原的浓度。与对照组相比，通过RIA和ELISA测定的糖尿病患者血清和尿液中IV型胶原的浓度有所升高。在患有大量蛋白尿或肾功能不全的糖尿病患者中，IV型胶原的血清和尿液浓度高于无肾病或有微量蛋白尿所确定的早期肾损伤的糖尿病患者。这些结果表明，晚期糖尿病肾病患者血清和尿液中IV型胶原的浓度升高。这些升高可能表明糖尿病患者基底膜代谢发生了改变。
