采用高灵敏度一步夹心酶免疫分析法检测糖尿病肾病患者尿Ⅳ型胶原的意义
Significance of urinary type IV collagen in patients with diabetic nephropathy using a highly sensitive one-step sandwich enzyme immunoassay.
作者信息
Yagame M, Suzuki D, Jinde K, Saotome N, Sato H, Noguchi M, Sakai H, Kuramoto T, Sekizuka K, Iijima T, Suzuki S, Tomino Y
机构信息
Department of Internal Medicine, School of Medicine, Tokai University, Kanagawa, Japan.
出版信息
J Clin Lab Anal. 1997;11(2):110-6. doi: 10.1002/(SICI)1098-2825(1997)11:2<110::AID-JCLA7>3.0.CO;2-F.
Urinary concentrations of type IV collagen in patients with diabetic nephropathy were measured by a highly sensitive, one-step sandwich enzyme immunoassay. Samples from 298 patients with noninsulin-dependent diabetes mellitus (NIDDM) and 80 healthy controls were examined. In diabetic patients with macroalbuminuria or renal insufficiency, the concentrations of urinary type IV collagen were significantly higher than those of diabetic patients with normoalbuminuria or healthy controls (P < 0.001). Urinary type IV collagen concentration in diabetic patients with microalbuminuria was significantly higher than that in diabetic patients with normoalbuminuria or that in healthy controls (P < 0.001). In contrast, there were no significant changes in the concentration of serum type IV collagen between microalbuminuric patients and normoalbuminuric patients. The area under the receiver operating characteristic (ROC) curve for the urinary type IV collagen concentration was equivalent to that of urinary albumin. It was concluded that urinary type IV collagen concentration determined using this method might be a useful marker for the early detection of diabetic nephropathy.
采用高灵敏度一步夹心酶免疫测定法检测糖尿病肾病患者尿中IV型胶原的浓度。对298例非胰岛素依赖型糖尿病（NIDDM）患者和80例健康对照者的样本进行了检测。在患有大量白蛋白尿或肾功能不全的糖尿病患者中，尿IV型胶原的浓度显著高于白蛋白尿正常的糖尿病患者或健康对照者（P<0.001）。微量白蛋白尿的糖尿病患者尿IV型胶原浓度显著高于白蛋白尿正常的糖尿病患者或健康对照者（P<0.001）。相比之下，微量白蛋白尿患者和正常白蛋白尿患者血清IV型胶原浓度无显著变化。尿IV型胶原浓度的受试者工作特征（ROC）曲线下面积与尿白蛋白相当。得出结论：用该方法测定的尿IV型胶原浓度可能是早期检测糖尿病肾病的有用标志物。
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