Zuccaro P, Altieri I, Rosa M, Passa A R, Pichini S, Pacifici R
Istituto Superiore di Sanità, Roma, Italy.
J Chromatogr B Biomed Appl. 1995 Jun 9;668(1):187-8. doi: 10.1016/0378-4347(95)00066-r.
A solid-phase extraction, using Extrelut-1 glass columns, has been applied to urine samples of both passive and active smokers for high-performance liquid chromatographic determination of nicotine and its metabolites cotinine and trans-3'-hydroxycotinine. Chromatography was performed using a reversed-phase LC8DB column and a mobile phase consisting of water-acetonitrile (80:9, v/v) containing 5 ml triethylamine, 670 mg/l sodium heptanesulphonate, and 0.034 M each of K2HPO4 and citric acid (pH 4.4), at a flow-rate of 1.6 ml/min. The results obtained indicate that solid-phase extraction is a reliable and quick procedure which can be applied also to other nicotine metabolites.
采用Extrelut - 1玻璃柱进行固相萃取，已应用于被动吸烟者和主动吸烟者的尿液样本，用于高效液相色谱法测定尼古丁及其代谢物可替宁和反式 - 3'-羟基可替宁。使用反相LC8DB柱进行色谱分析，流动相由水 - 乙腈（80:9，v/v）组成，其中含有5 ml三乙胺、670 mg/l庚烷磺酸钠以及0.034 M的磷酸氢二钾和柠檬酸（pH 4.4），流速为1.6 ml/min。所得结果表明，固相萃取是一种可靠且快速的方法，也可应用于其他尼古丁代谢物。
