Cornillie F J, Lauweryns J M, Seppälä M, Riittinen L, Koninckx P R
Department of Obstetrics and Gynaecology, U.Z. Gasthuisberg, Leuven, Finland.
Hum Reprod. 1991 Nov;6(10):1411-5. doi: 10.1093/oxfordjournals.humrep.a137280.
Localization and immunohistochemical staining patterns of endometrial protein PP14 were studied in 55 patients providing 86 histologically confirmed pelvic and ovarian endometriotic implants. The cellular localization of PP14 in endometriotic implants is comparable with that in the endometrium: epithelial cells express PP14, whereas stromal cells are negative. Positive immunostaining is restricted to apical secretory-like granules of the epithelial cells. In endometriotic implants with positive staining, PP14 is expressed by some, but not all, glandular epithelial cells. Furthermore, positive staining for PP14 is observed most frequently in foci with in-situ secretory differentiation, whereas implants with proliferative or atrophic implants only rarely express PP14. Finally, PP14 can be localized in endometriotic foci at different depths of infiltration, although positive labelling is seen more often in superficial implants. These data demonstrate that PP14 can be expressed by endometriotic glandular epithelial cells with secretory cellular differentiation and that the histological appearance of ectopic implants sometimes only poorly reflects their function.
对55例患者的86个经组织学证实的盆腔和卵巢子宫内膜异位种植体进行了子宫内膜蛋白PP14的定位及免疫组化染色模式研究。PP14在子宫内膜异位种植体中的细胞定位与在子宫内膜中的定位相似：上皮细胞表达PP14，而基质细胞呈阴性。阳性免疫染色局限于上皮细胞的顶端分泌样颗粒。在呈阳性染色的子宫内膜异位种植体中，部分而非全部腺上皮细胞表达PP14。此外，PP14阳性染色最常见于原位分泌分化的病灶，而增殖性或萎缩性种植体很少表达PP14。最后，PP14可定位在不同浸润深度的子宫内膜异位病灶中，尽管在浅表种植体中阳性标记更常见。这些数据表明，PP14可由具有分泌细胞分化的子宫内膜异位腺上皮细胞表达，而异位种植体的组织学表现有时只能很差地反映其功能。
