Perazzi B, Menghi C, Coppolillo E, Gatta C, Cora Eliseht M, Vay C, Méndez O, Malamud de Rudavega H, de Torres R, Famiglietti A
Laboratorio de Bacteriología Clínica, Departamento de Bioquímica Clínica, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. Av. Córdoba 2351 (1120) Ciudad Autónoma de Buenos Aires, Argentina.
Rev Argent Microbiol. 2007 Apr-Jun;39(2):99-104.
The aim of this study was to conduct a survey regarding the prevalence of trichomoniasis in pregnant patients and to evaluate the utility of different diagnostic methods. Two hundred and twenty three vaginal swab specimens from pregnant women were prospectively examined. Trichomonas vaginalis was investigated by various microscopic examinations, solid culture medium and liquid culture medium. The sensitivity and specificity of microscopy were evaluated by considering both culture media as the "gold standards". The prevalence of T. vaginalis obtained by both culture media (liquid plus solid media) was 4.5% (10/223). The prevalence of T. vaginalis obtained by direct smear, May-Grunwald Giemsa staining, sodium acetate-acetic acid-formalin (SAF)/Methylene blue staining-fixing technique, solid medium and liquid medium was 1.3%, 1.8%, 1.8% and 4.5%, respectively. The sensitivity of the direct smear was 30 %, but for the May-Grunwald Giemsa staining and the SAF/Methylene blue staining-fixing technique was 40%. Considering the three microscopic examinations altogether, the sensitivity rose to 50% and the specificity was 100% for all of them. The solid medium detected only 50% of the positive cases; the liquid medium detected 100%. Due to the low sensitivity obtained with microscopy in asymptomatic pregnant patients, we recommend the use of the liquid medium during pregnancy, in order to provide an early treatment.
本研究的目的是对孕妇滴虫病的患病率进行调查，并评估不同诊断方法的效用。前瞻性地检查了223例孕妇的阴道拭子标本。通过各种显微镜检查、固体培养基和液体培养基对阴道毛滴虫进行检测。通过将两种培养基均视为“金标准”来评估显微镜检查的敏感性和特异性。两种培养基（液体加固体培养基）检测到的阴道毛滴虫患病率为4.5%（10/223）。直接涂片、May-Grunwald Giemsa染色、醋酸钠-醋酸-甲醛（SAF）/亚甲蓝染色固定技术、固体培养基和液体培养基检测到的阴道毛滴虫患病率分别为1.3%、1.8%、1.8%和4.5%。直接涂片的敏感性为30%，但May-Grunwald Giemsa染色和SAF/亚甲蓝染色固定技术的敏感性为40%。将这三种显微镜检查方法综合考虑，敏感性升至50%，特异性均为100%。固体培养基仅检测到50%的阳性病例；液体培养基检测到100%。由于无症状孕妇显微镜检查的敏感性较低，我们建议在孕期使用液体培养基，以便进行早期治疗。
