Gao Min, Huang Wei, Liu Chun-Zhao
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Oct 15;858(1-2):22-6. doi: 10.1016/j.jchromb.2007.07.046. Epub 2007 Aug 7.
Scutellarin, a flavone glycoside, popularly used in the treatment of heart disease, has been efficiently separated using macroporous resins from crude extracts of Chinese medicinal plant Erigeron breviscapus (vant.) Hand. Mazz. HPD-800 resin offered the best adsorption and desorption capacity for scutellarin among the eight macroporous resins tested, and its adsorption data at 25 degrees C fit best to the Langmuir isotherm. The dynamic adsorption and desorption experiments have been carried out on a HPD-800 resin packed column to optimize the separation process of scutellarin from the crude extracts of E. breviscapus. After one run treatment with HPD-800 resin, the scutellarin content in the product was increased 15.69-fold from 2.61% to 40.96% with a recovery yield of 95.01%. The preparative separation process via adsorption-desorption method developed in this study provides a new approach for scale-up separation and purification of scutellarin for its wide pharmaceutical use.
灯盏花素，一种黄酮苷，常用于治疗心脏病，已通过大孔树脂从中药植物短葶飞蓬（Erigeron breviscapus (vant.) Hand. Mazz.）的粗提物中有效分离出来。在所测试的八种大孔树脂中，HPD - 800树脂对灯盏花素具有最佳的吸附和解吸能力，其在25℃下的吸附数据最符合朗缪尔等温线。在填充有HPD - 800树脂的柱上进行了动态吸附和解吸实验，以优化从短葶飞蓬粗提物中分离灯盏花素的工艺。用HPD - 800树脂进行一次处理后，产品中灯盏花素的含量从2.61%提高到40.96%，提高了15.69倍，回收率为95.01%。本研究开发的通过吸附 - 解吸方法的制备分离工艺为灯盏花素的大规模分离和纯化提供了一种新方法，因其具有广泛的药用价值。
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