Liu Xin, Wang Jianming, Zhou Changxin, Gan Lishe
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
J Biomed Biotechnol. 2010;2010:572570. doi: 10.1155/2010/572570. Epub 2010 Dec 27.
Syringopicroside is the major constituent in Folium syringae leaves with known pharmacological activities. In this study, a simple method for preparative separation of syringopicroside from F. syringae leaves with macroporous resins was developed. Adsorption characteristics of syringopicroside on six types of macroporous resins, including ADS-8, ADS-17, D141, NKA-9, HPD450, and HPD600, have been compared, among which D141 resin showed the best adsorption and desorption capacities for syringopicroside. Adsorption isotherms were used to D141 resin at different temperatures and fitted well to Langmuir and Freundlich equations. Dynamic adsorption and desorption tests were performed on D141 resin-packed column to optimize the separation process of syringopicroside. After one run with D141 resin, the content of syringopicroside was increased 24-fold from 2.32% to 55.74% with a recovery yield of 92.16%. The chromatographic process optimized in this work avoids toxic organic solvent and, thus, is a promising basis for large-scale preparation of syringopicroside.
丁香苦苷是丁香叶中的主要成分，具有已知的药理活性。在本研究中，开发了一种用大孔树脂从丁香叶中制备分离丁香苦苷的简单方法。比较了丁香苦苷在六种大孔树脂（包括ADS - 8、ADS - 17、D141、NKA - 9、HPD450和HPD600）上的吸附特性，其中D141树脂对丁香苦苷表现出最佳的吸附和解吸能力。在不同温度下对D141树脂进行吸附等温线研究，其与Langmuir和Freundlich方程拟合良好。在填充D141树脂的柱上进行动态吸附和解吸试验，以优化丁香苦苷的分离过程。用D141树脂进行一次分离后，丁香苦苷的含量从2.32%提高到55.74%，提高了24倍，回收率为92.16%。本工作中优化的色谱过程避免了使用有毒有机溶剂，因此是大规模制备丁香苦苷的有前景的基础。
