Ishak Marluísa de Oliveira Guimarães, Martins Rosimar Neris, Machado Paula Renata Lima, de Souza Lia Lobato Batista, Machado Luiz Fernando Almeida, Azevedo Vânia Nakauth, Katano Harutaka, Sata Tetsutaro, Hasegawa Hideki, Vallinoto Antonio Carlos Rosário, Ishak Ricardo
Virus Laboratory, Universidade Federal do Para, Brazil.
J Med Virol. 2007 Oct;79(10):1537-44. doi: 10.1002/jmv.20995.
The present study describes the molecular epidemiology of Human herpesvirus 8 (HHV-8) among four Indian tribes (Kararao, Arara Laranjal, Tiriyo, and Zo'e) of the Amazon region of Brazil and a group of HIV-1-infected subjects from the urban population of Belem, Para. Infection was characterized by the presence of antibodies using ELISA (measuring antibodies to ORF59, ORF65, K8.1A, K8.1B, and ORF73), and molecular assays (gene amplification of the regions ORF26 and the variable region VR1). Antibodies to HHV-8 were detected in 66 samples of the 221 Brazilian Amerindians, namely, 6 (25%) in the Kararao, 18 (19.6%) in the Arara Laranjal, 24 (42.9%) in the Tiriyo, and 18 (36.7%) in the Zo'e. Among the 477 HIV-1-infected subjects, antibodies to HHV-8 were present in 74 (15.5%) persons. The ORF26 region was amplified in seven samples, one of the Arara Laranjal, one of the Tiriyo, two of the Zo'e, and three of the HIV-1-infected group. Subtyping of HHV-8 described a high multiplicity of molecular subtypes, including C (Zo'e), E (Tiriyo), and B (HIV-1 infected). Serological results confirm the high prevalence of HHV-8 among Amerindians and the presence of three subtypes in the Amazon region of Brazil, including a unique subtype, which favors the idea of HHV-8 as an ancient human infection within this particular geographical region.
本研究描述了巴西亚马逊地区四个印第安部落（卡拉劳、阿拉拉兰贾尔、蒂里约和佐埃）以及帕拉州贝伦市城市人口中一组感染HIV-1的人群中人类疱疹病毒8型（HHV-8）的分子流行病学情况。通过酶联免疫吸附测定法（检测针对开放阅读框59、开放阅读框65、K8.1A、K8.1B和开放阅读框73的抗体）和分子检测方法（开放阅读框26区域和可变区VR1的基因扩增）来确定感染情况。在221名巴西美洲印第安人的66份样本中检测到了HHV-8抗体，其中卡拉劳部落有6份（25%），阿拉拉兰贾尔部落有18份（19.6%），蒂里约部落有24份（42.9%），佐埃部落有18份（36.7%）。在477名感染HIV-1的受试者中，有74人（15.5%）检测到了HHV-8抗体。在7份样本中扩增出了开放阅读框26区域，其中阿拉拉兰贾尔部落1份，蒂里约部落1份，佐埃部落2份，感染HIV-1的人群中有3份。HHV-8的亚型分析显示存在多种分子亚型，包括C型（佐埃部落）、E型（蒂里约部落）和B型（感染HIV-1的人群）。血清学结果证实了HHV-8在美洲印第安人中的高流行率以及巴西亚马逊地区存在三种亚型，包括一种独特的亚型，这支持了HHV-8是该特定地理区域内一种古老人类感染的观点。
