Machado Paula Renata Lima, Farias Kleber Juvenal Silva, Pereira Maira Gabriela Martins, Freitas Patrícia Pereira da Silva de, Fonseca Benedito Antônio Lopes da
PhD. Biomedical Scientist, Virology Research Center, Faculdade de Medicina de Ribeirão Preto (FMRP), Universidade de São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
MSc. Biologist, Virology Research Center, Faculdade de Medicina de Ribeirão Preto (FMRP), Universidade de São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
Sao Paulo Med J. 2015 Apr 14;134(3):187-92. doi: 10.1590/1516-3180.2014.8973010.
Kaposi's sarcoma (KS) is a common neoplastic disease in AIDS patients. The aim of this study was to evaluate the frequency of human herpesvirus 8 (HHV-8) infection in human immunodeficiency virus (HIV)-infected patients, with or without KS manifestations and correlate HHV-8 detection with KS staging.
Analytic cross-sectional study conducted in a public tertiary-level university hospital in Ribeirão Preto, São Paulo, Brazil.
Antibodies against HHV-8 lytic-phase antigens were detected by means of the immunofluorescence assay. HHV-8 DNA was detected in the patient samples through a nested polymerase chain reaction (nested PCR) that amplified a region of open reading frame (ORF)-26 of HHV-8.
Anti-HHV-8 antibodies were detected in 30% of non-KS patients and 100% of patients with KS. Furthermore, the HHV-8 DNA detection rates observed in HIV-positive patients with KS were 42.8% in serum, 95.4% in blood samples and 100% in skin biopsies; and in patients without KS, the detection rate was 4% in serum. Out of the 16 serum samples from patients with KS-AIDS who were classified as stage II, two were positive (12.5%); and out of the 33 samples from patients in stage IV, 19 (57.6%) were positive.
We observed an association between HHV-8 detection and disease staging, which was higher in the serum of patients in stage IV. This suggests that detection of HHV-8 DNA in serum could be very useful for clinical assessment of patients with KS and for monitoring disease progression.
卡波西肉瘤（KS）是艾滋病患者中常见的肿瘤性疾病。本研究旨在评估人类免疫缺陷病毒（HIV）感染患者中人类疱疹病毒8型（HHV-8）感染的频率，无论有无KS表现，并将HHV-8检测结果与KS分期相关联。
在巴西圣保罗州里贝朗普雷图的一家公立三级大学医院进行的分析性横断面研究。
通过免疫荧光法检测针对HHV-8裂解期抗原的抗体。通过巢式聚合酶链反应（巢式PCR）在患者样本中检测HHV-8 DNA，该反应扩增HHV-8开放阅读框（ORF）-26的一个区域。
在30%的非KS患者和100%的KS患者中检测到抗HHV-8抗体。此外，在KS的HIV阳性患者中观察到的HHV-8 DNA检测率在血清中为42.8%，在血液样本中为95.4%，在皮肤活检中为100%；而在无KS的患者中，血清检测率为4%。在16例被分类为II期的KS-艾滋病患者的血清样本中，有2例呈阳性（12.5%）；在33例IV期患者的样本中，有19例（57.6%）呈阳性。
我们观察到HHV-8检测与疾病分期之间存在关联，IV期患者血清中的关联更高。这表明血清中HHV-8 DNA的检测对于KS患者的临床评估和疾病进展监测可能非常有用。
