Starkuviene Vytaute, Pepperkok Rainer, Erfle Holger
European Molecular Biology Laboratory, Cell Biology & Cell Biophysics Unit, Heidelberg, Germany.
Expert Rev Proteomics. 2007 Aug;4(4):479-89. doi: 10.1586/14789450.4.4.479.
Transfected cell microarrays are considered to be a breakthrough methodology for high-throughput and high-content functional genomics. Here, recent advances in the cell microarray field are reviewed, along with its potential to increase the speed of determining gene function. These advances, combined with an increasing number and diversity of gene perturbing systems, such as RNAi and ectopic gene expression, provide tools for expanding our understanding of biology at the systems level.
转染细胞微阵列被认为是高通量和高内涵功能基因组学的一项突破性技术。本文综述了细胞微阵列领域的最新进展,以及其在加快基因功能确定速度方面的潜力。这些进展,再加上诸如RNA干扰和异位基因表达等越来越多且多样的基因干扰系统,为我们在系统层面拓展对生物学的理解提供了工具。