细胞微阵列和RNA干扰技术逐步揭示基因功能。

Cell microarrays and RNA interference chip away at gene function.

作者信息

Wheeler Douglas B, Carpenter Anne E, Sabatini David M

机构信息

Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Department of Biology, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA.

出版信息

Nat Genet. 2005 Jun;37 Suppl:S25-30. doi: 10.1038/ng1560.

DOI:10.1038/ng1560

PMID:15920526

Abstract

The recent development of cell microarrays offers the potential to accelerate high-throughput functional genetic studies. The widespread use of RNA interference (RNAi) has prompted several groups to fabricate RNAi cell microarrays that make possible discrete, in-parallel transfection with thousands of RNAi reagents on a microarray slide. Though still a budding technology, RNAi cell microarrays promise to increase the efficiency, economy and ease of genome-wide RNAi screens in metazoan cells.

摘要

细胞微阵列技术的最新进展为加速高通量功能基因研究提供了可能。RNA干扰（RNAi）的广泛应用促使多个研究小组制造了RNAi细胞微阵列，使得在微阵列载玻片上能够对数千种RNAi试剂进行离散的平行转染。尽管RNAi细胞微阵列技术仍处于萌芽阶段，但它有望提高后生动物细胞全基因组RNAi筛选的效率、经济性和便捷性。