JBP2是一种类似于SWI2/SNF2的蛋白质，它在布氏锥虫的血流形式中调节端粒DNA的从头糖基化。
JBP2, a SWI2/SNF2-like protein, regulates de novo telomeric DNA glycosylation in bloodstream form Trypanosoma brucei.
作者信息
Kieft Rudo, Brand Verena, Ekanayake Dilrukshi K, Sweeney Kate, DiPaolo Courtney, Reznikoff William S, Sabatini Robert
机构信息
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.
出版信息
Mol Biochem Parasitol. 2007 Nov;156(1):24-31. doi: 10.1016/j.molbiopara.2007.06.010. Epub 2007 Jun 28.
Abstract
Synthesis of the modified thymine base, beta-d-glucosyl-hydroxymethyluracil or J, within telomeric DNA of Trypanosoma brucei correlates with the bloodstream form specific epigenetic silencing of telomeric variant surface glycoprotein genes involved in antigenic variation. In order to analyze the function of base J in the regulation of antigenic variation, we are characterizing the regulatory mechanism of J biosynthesis. We have recently proposed a model in which chromatin remodeling by a SWI2/SNF2-like protein (JBP2) regulates the developmental and de novo site-specific localization of J synthesis within bloodstream form trypanosome DNA. Consistent with this model, we now show that JBP2 (-/-) bloodstream form trypanosomes contain five-fold less base J and are unable to stimulate de novo J synthesis in newly generated telomeric arrays.
摘要
在布氏锥虫的端粒DNA中，修饰胸腺嘧啶碱基β - d - 葡糖基羟甲基尿嘧啶（即J）的合成与参与抗原变异的端粒可变表面糖蛋白基因的血流形式特异性表观遗传沉默相关。为了分析碱基J在抗原变异调控中的功能，我们正在对J生物合成的调控机制进行表征。我们最近提出了一个模型，其中一种类似SWI2/SNF2的蛋白（JBP2）介导的染色质重塑调节了血流形式锥虫DNA中J合成的发育和从头位点特异性定位。与该模型一致，我们现在表明JBP2（-/-）血流形式锥虫中的碱基J含量减少了五倍，并且无法刺激新生成的端粒阵列中的J从头合成。
相似文献
1
JBP2, a SWI2/SNF2-like protein, regulates de novo telomeric DNA glycosylation in bloodstream form Trypanosoma brucei.JBP2是一种类似于SWI2/SNF2的蛋白质，它在布氏锥虫的血流形式中调节端粒DNA的从头糖基化。
Mol Biochem Parasitol. 2007 Nov;156(1):24-31. doi: 10.1016/j.molbiopara.2007.06.010. Epub 2007 Jun 28.
2
Regulation of trypanosome DNA glycosylation by a SWI2/SNF2-like protein.一种SWI2/SNF2样蛋白对锥虫DNA糖基化的调控
Mol Cell. 2005 Feb 4;17(3):441-51. doi: 10.1016/j.molcel.2004.12.022.
3
Localization of the modified base J in telomeric VSG gene expression sites of Trypanosoma brucei.修饰碱基J在布氏锥虫端粒VSG基因表达位点中的定位
Genes Dev. 1997 Dec 1;11(23):3232-41. doi: 10.1101/gad.11.23.3232.
4
JBP1 and JBP2 are two distinct thymidine hydroxylases involved in J biosynthesis in genomic DNA of African trypanosomes.JBP1和JBP2是两种不同的胸苷羟化酶，参与非洲锥虫基因组DNA中J的生物合成。
Nucleic Acids Res. 2009 Apr;37(5):1452-62. doi: 10.1093/nar/gkn1067. Epub 2009 Jan 9.
5
Two thymidine hydroxylases differentially regulate the formation of glucosylated DNA at regions flanking polymerase II polycistronic transcription units throughout the genome of Trypanosoma brucei.两种胸苷羟化酶在调控布氏冈比亚锥虫基因组中转录酶 II 多顺反子转录单位侧翼区域的糖基化 DNA 形成方面存在差异。
Nucleic Acids Res. 2010 Jul;38(12):3923-35. doi: 10.1093/nar/gkq146. Epub 2010 Mar 9.
6
beta-D-glucosyl-hydroxymethyluracil, a novel base in African trypanosomes and other Kinetoplastida.β-D-葡萄糖基羟甲基尿嘧啶，一种在非洲锥虫和其他动基体目生物中的新型碱基。
Mol Biochem Parasitol. 1997 Dec 1;90(1):1-8. doi: 10.1016/s0166-6851(97)00170-9.
7
The modified base J is the target for a novel DNA-binding protein in kinetoplastid protozoans.修饰碱基J是动质体原生动物中一种新型DNA结合蛋白的作用靶点。
EMBO J. 1999 Nov 15;18(22):6573-81. doi: 10.1093/emboj/18.22.6573.
8
Biosynthesis and function of the modified DNA base beta-D-glucosyl-hydroxymethyluracil in Trypanosoma brucei.布氏锥虫中修饰的DNA碱基β-D-葡萄糖基羟甲基尿嘧啶的生物合成与功能
Mol Cell Biol. 1998 Oct;18(10):5643-51. doi: 10.1128/MCB.18.10.5643.
9
Tandemly repeated DNA is a target for the partial replacement of thymine by beta-D-glucosyl-hydroxymethyluracil in Trypanosoma brucei.串联重复DNA是布氏锥虫中β-D-葡糖基羟甲基尿嘧啶部分取代胸腺嘧啶的靶点。
Mol Biochem Parasitol. 2000 Jul;109(2):133-45. doi: 10.1016/s0166-6851(00)00247-4.
10
J-binding protein increases the level and retention of the unusual base J in trypanosome DNA.J结合蛋白可提高锥虫DNA中异常碱基J的水平及保留率。
Mol Microbiol. 2002 Oct;46(1):37-47. doi: 10.1046/j.1365-2958.2002.03144.x.
引用本文的文献
1
Behind Base J: The Roles of JBP1 and JBP2 on Trypanosomatids.碱基J背后：JBP1和JBP2在锥虫中的作用
Pathogens. 2023 Mar 16;12(3):467. doi: 10.3390/pathogens12030467.
2
The SPARC complex defines RNAPII promoters in .SPARC 复合物在. 中定义了 RNAPII 启动子。
Elife. 2022 Sep 28;11:e83135. doi: 10.7554/eLife.83135.
3
Carbohydrate biomarkers for future disease detection and treatment.用于未来疾病检测与治疗的碳水化合物生物标志物。
Sci China Chem. 2010;53(1):3-20. doi: 10.1007/s11426-010-0021-3. Epub 2010 Feb 7.
4
Identification of a novel base J binding protein complex involved in RNA polymerase II transcription termination in trypanosomes.鉴定一种新型碱基 J 结合蛋白复合物，该复合物参与锥虫 RNA 聚合酶 II 转录终止。
PLoS Genet. 2020 Feb 21;16(2):e1008390. doi: 10.1371/journal.pgen.1008390. eCollection 2020 Feb.
5
A method for the efficient and selective identification of 5-hydroxymethyluracil in genomic DNA.一种在基因组DNA中高效且选择性地鉴定5-羟甲基尿嘧啶的方法。
Biol Methods Protoc. 2017 Jan;2(1). doi: 10.1093/biomethods/bpw006. Epub 2017 Jan 25.
6
Defining the sequence requirements for the positioning of base J in DNA using SMRT sequencing.使用单分子实时测序（SMRT测序）确定DNA中碱基J定位的序列要求。
Nucleic Acids Res. 2015 Feb 27;43(4):2102-15. doi: 10.1093/nar/gkv095. Epub 2015 Feb 6.
7
Regulation of transcription termination by glucosylated hydroxymethyluracil, base J, in Leishmania major and Trypanosoma brucei.利什曼原虫和布氏锥虫中糖基化羟甲基尿嘧啶（碱基J）对转录终止的调控
Nucleic Acids Res. 2014 Sep;42(15):9717-29. doi: 10.1093/nar/gku714. Epub 2014 Aug 7.
8
Quantitative mass spectrometry-based analysis of β-D-glucosyl-5-hydroxymethyluracil in genomic DNA of Trypanosoma brucei.基于定量质谱法分析布氏锥虫基因组DNA中的β-D-葡萄糖基-5-羟甲基尿嘧啶。
J Am Soc Mass Spectrom. 2014 Oct;25(10):1763-70. doi: 10.1007/s13361-014-0960-6. Epub 2014 Jul 31.
9
Identification of the glucosyltransferase that converts hydroxymethyluracil to base J in the trypanosomatid genome.在锥虫基因组中鉴定将羟甲基尿嘧啶转化为碱基J的葡糖基转移酶。
J Biol Chem. 2014 Jul 18;289(29):20273-82. doi: 10.1074/jbc.M114.579821. Epub 2014 Jun 2.
10
JBP1 and JBP2 proteins are Fe2+/2-oxoglutarate-dependent dioxygenases regulating hydroxylation of thymidine residues in trypanosome DNA.JBP1 和 JBP2 蛋白是依赖 Fe2+/2-氧代戊二酸的双加氧酶，可调节原生动物 DNA 中胸苷残基的羟化。
J Biol Chem. 2012 Jun 8;287(24):19886-95. doi: 10.1074/jbc.M112.341974. Epub 2012 Apr 18.
本文引用的文献
1
Repression of polymerase I-mediated gene expression at Trypanosoma brucei telomeres.布氏锥虫端粒处聚合酶I介导的基因表达的抑制
EMBO Rep. 2006 Jan;7(1):93-9. doi: 10.1038/sj.embor.7400575.
2
Regulation of trypanosome DNA glycosylation by a SWI2/SNF2-like protein.一种SWI2/SNF2样蛋白对锥虫DNA糖基化的调控
Mol Cell. 2005 Feb 4;17(3):441-51. doi: 10.1016/j.molcel.2004.12.022.
3
J-binding protein increases the level and retention of the unusual base J in trypanosome DNA.J结合蛋白可提高锥虫DNA中异常碱基J的水平及保留率。
Mol Microbiol. 2002 Oct;46(1):37-47. doi: 10.1046/j.1365-2958.2002.03144.x.
4
Expression of the human DNA glycosylase hSMUG1 in Trypanosoma brucei causes DNA damage and interferes with J biosynthesis.人类DNA糖基化酶hSMUG1在布氏锥虫中的表达会导致DNA损伤并干扰J生物合成。
Nucleic Acids Res. 2002 Sep 15;30(18):3919-26. doi: 10.1093/nar/gkf533.
5
Recognition of base J in duplex DNA by J-binding protein.J结合蛋白对双链DNA中碱基J的识别。
J Biol Chem. 2002 Jan 11;277(2):958-66. doi: 10.1074/jbc.M109000200. Epub 2001 Nov 7.
6
The modified base J is the target for a novel DNA-binding protein in kinetoplastid protozoans.修饰碱基J是动质体原生动物中一种新型DNA结合蛋白的作用靶点。
EMBO J. 1999 Nov 15;18(22):6573-81. doi: 10.1093/emboj/18.22.6573.
7
Biosynthesis and function of the modified DNA base beta-D-glucosyl-hydroxymethyluracil in Trypanosoma brucei.布氏锥虫中修饰的DNA碱基β-D-葡萄糖基羟甲基尿嘧啶的生物合成与功能
Mol Cell Biol. 1998 Oct;18(10):5643-51. doi: 10.1128/MCB.18.10.5643.
8
beta-D-glucosyl-hydroxymethyluracil, a novel base in African trypanosomes and other Kinetoplastida.β-D-葡萄糖基羟甲基尿嘧啶，一种在非洲锥虫和其他动基体目生物中的新型碱基。
Mol Biochem Parasitol. 1997 Dec 1;90(1):1-8. doi: 10.1016/s0166-6851(97)00170-9.
9
beta-D-glucosyl-hydroxymethyluracil is a conserved DNA modification in kinetoplastid protozoans and is abundant in their telomeres.β-D-葡糖基羟甲基尿嘧啶是动质体原生动物中一种保守的DNA修饰，在其端粒中含量丰富。
Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2366-71. doi: 10.1073/pnas.95.5.2366.
10
Localization of the modified base J in telomeric VSG gene expression sites of Trypanosoma brucei.修饰碱基J在布氏锥虫端粒VSG基因表达位点中的定位
Genes Dev. 1997 Dec 1;11(23):3232-41. doi: 10.1101/gad.11.23.3232.
Zotero 插件