Maclean Leann, Perry Malcolm B, Nossova Ludmila, Kaplan Heidi, Vinogradov Evgeny
Institute for Biological Sciences, National Research Council, 100 Sussex Dr., Ottawa, ON, Canada K1A 0R6.
Carbohydr Res. 2007 Nov 26;342(16):2474-80. doi: 10.1016/j.carres.2007.07.023. Epub 2007 Aug 7.
Gram-negative rod shaped bacterium Myxococcus xanthus DK1622 produces a smooth-type LPS. The structure of the polysaccharide O-chain and the core-lipid A region of the LPS has been determined by chemical and spectroscopic methods. The O-chain was built up of disaccharide repeating units having the following structure: -->6)-alpha-D-Glcp-(1-->4)-alpha-D-GalpNAc6oMe*-(1--> with partially methylated GalNAc residue. The core region consisted of a phosphorylated hexasaccharide, containing one Kdo residue, unsubstituted at O-4, and no heptose residues. The lipid A component consisted of beta-GlcN-(1-->6)-alpha-GlcN1P disaccharide, N-acylated with 13-methyl-C14-3OH (iso-C15-3OH), C16-3OH, and 15-methyl-C16-3OH (iso-C17-3OH) acids. The lipid portion contained O-linked iso-C16 acid.
革兰氏阴性杆状细菌黄色粘球菌DK1622产生一种光滑型脂多糖。脂多糖的多糖O链和核心脂质A区域的结构已通过化学和光谱方法确定。O链由具有以下结构的二糖重复单元组成：→6)-α-D-葡萄糖-(1→4)-α-D-半乳糖胺6-O-甲基*-(1→，其中半乳糖胺残基部分甲基化。核心区域由一个磷酸化的六糖组成，含有一个在O-4位未取代且没有庚糖残基的Kdo残基。脂质A成分由β-葡萄糖胺-(1→6)-α-葡萄糖胺1-磷酸二糖组成，用13-甲基-C14-3-OH（异-C15-3-OH）、C16-3-OH和15-甲基-C16-3-OH（异-C17-3-OH）酸进行N-酰化。脂质部分含有O-连接的异-C16酸。
