Dobie D J, Miller M A, Urban J H, Raskind M A, Dorsa D M
Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle 98195.
Neurobiol Aging. 1991 Sep-Oct;12(5):419-23. doi: 10.1016/0197-4580(91)90067-t.
To determine whether aging influences arginine vasopressin (AVP) biosynthesis in the extrahypothalamic neurons of the bed nucleus of the stria terminalis (BNST), we used in situ hybridization and quantitative autoradiography to compare AVP mRNA in 3-month-old, 14-month-old, and 24-month-old male Fischer 344 rats. As AVP synthesis in the BNST has previously been shown to be steroid-dependent, plasma testosterone (T) was measured by radioimmunoassay. The 24-month-old animals had significantly fewer AVP-labelled cells than either the 3-month-old (p less than 0.01) or 14-month-old (p less than 0.05) animals. The cells that were present in the 24-month animals were less intensely labelled than in the other groups, as indicated by a significantly reduced number of grains per cell (p less than 0.01). Plasma T was also significantly lower in 24-month-old animals when compared with 3-month (p less than 0.01) or 14-month (p less than 0.05) groups. The results indicate that there is a marked age-related decline in vasopressin biosynthetic activity in neurons of the BNST.
为了确定衰老是否会影响终纹床核（BNST）下丘脑外神经元中精氨酸加压素（AVP）的生物合成，我们使用原位杂交和定量放射自显影技术，比较了3个月大、14个月大和24个月大的雄性Fischer 344大鼠的AVP mRNA。由于先前已证明BNST中的AVP合成是类固醇依赖性的，因此通过放射免疫测定法测量了血浆睾酮（T）。与3个月大的动物（p<0.01）或14个月大的动物（p<0.05）相比，24个月大的动物中AVP标记的细胞明显更少。24个月大动物中存在的细胞标记强度低于其他组，这表现为每个细胞的颗粒数量显著减少（p<0.01）。与3个月（p<0.01）或14个月（p<0.05）组相比，24个月大动物的血浆T也显著降低。结果表明，BNST神经元中加压素生物合成活性存在明显的年龄相关下降。
