Szot P, Dorsa D M
Geriatric Research, Education and Clinical Center (GRECC), Veterans Affairs Medical Center, Seattle, WA 98108.
Brain Res Dev Brain Res. 1993 Jun 8;73(2):177-83. doi: 10.1016/0165-3806(93)90136-x.
The ontogeny of vasopressin (VP) gene expression in extrahypothalamic neurons of the bed nucleus of the stria terminalis (BNST) and medial amygdala (MA) was investigated using in situ hybridization. In both the BNST and MA, VP mRNA-positive cell profiles were only detected a few days after birth. In males, VP mRNA-expressing cell profiles appear in the BNST on day 3 and in the MA on day 5. In contrast, hybridization signals could not be detected in the female brain until day 14 in the BNST and day 35 in the MA. Adult VP mRNA levels are attained by day 35 in the BNST and day 60 in the MA for both sexes. From this data, it is apparent that development of the VP system is delayed in females compared to the males. Therefore, these data suggest that the sexual dimorphic pattern in the expression of VP mRNA in these nuclei of the adult brain is evident early in the development process.
利用原位杂交技术研究了终纹床核（BNST）和内侧杏仁核（MA）下丘脑外神经元中加压素（VP）基因表达的个体发生。在BNST和MA中，出生后几天才检测到VP mRNA阳性细胞形态。在雄性中，表达VP mRNA的细胞形态在出生后第3天出现在BNST，第5天出现在MA。相比之下，在雌性大脑中，直到出生后第14天在BNST和第35天在MA才能检测到杂交信号。两性在BNST中到出生后第35天、在MA中到出生后第60天达到成年VP mRNA水平。从这些数据可以明显看出，与雄性相比，雌性VP系统的发育延迟。因此，这些数据表明，成年大脑这些核团中VP mRNA表达的性二态模式在发育过程早期就很明显。
